碘过量对成年大鼠大脑皮质和海马形态学影响的实验研究

发布时间：2018-01-16 19:09

本文关键词：碘过量对成年大鼠大脑皮质和海马形态学影响的实验研究　出处：《天津医科大学》2005年硕士论文　论文类型：学位论文

更多相关文章： 碘过量 碘缺乏 NSE GFAP

【摘要】：目的观察Wistar成年大鼠在不同浓度碘水喂养3个月和6个月后,大脑皮质内锥体细胞层锥体细胞和海马神经元及神经胶质细胞的形态学改变,进一步探讨碘过量对成年大鼠大脑皮质及海马功能的影响。方法断乳一月Wistar大鼠(60日龄),雌雄各半,随机分为6组:低碘组(LI组),适碘组(NI组),5倍碘组(5HI组),10倍碘组(10HI组),50倍碘组(50HI组)和100倍碘组(100HI组)。LI组饲以低碘地区粮食配制的饲料(平均含碘量为50μg/kg),饮用去离子水;NI组和各高碘组均饲以标准的正常饲料(平均含碘量为300μg/kg),NI组饮用自来水(含碘量为5μgI~-/L),各高碘组分别饮用不同浓度的KI自来水,按每日进食量和饮水量计算各高碘组分别摄入碘量为NI组的5倍、10倍、50倍和100倍。观察喂养3个月和6个月后全脑重量及大脑皮质内锥体细胞层锥体细胞和海马神经元及神经胶质细胞的形态学改变(HE染色、免疫组化染色和镀银染色),并对NSE阳性神经元和GFAP阳性神经胶质细胞的体积密度(Vv),面数密度(N_A)、细胞质的平均灰度值及大脑皮质内锥体细胞层锥体细胞基底树突数进行形态计量学分析。结果 1.实验3个月和6个月各高碘组与同月份NI组比较,全脑绝对重量、相对重量无明显差异,6个月LI组与同月份NI组比较,全脑绝对重量明显降低(P0.05),但相对重量无明显差异。2.对海马研究结果:实验3个月和6个月各高碘组与同月份NI组比较,海马CA3区NSE阳性神经元Vv、N_A无明显差异,但细胞质阳性反应程度不同,经形态计量学分析,实验3个月和6个月100HI组与同月份NI组比较(P0.01)、实验6个月50HI组与NI组比较(P0.05)NSE阳性神经元细胞质的平均灰度值明显降低,实验6个月LI组与NI组比较(P0.01)NSE阳性神经元细胞质的平均灰度值也呈明显降低;实验3个月各高碘组、实验6个月LI组及各高碘组与同月份NI组
[Abstract]:Objective to observe the morphological changes of pyramidal cell layer pyramidal cells, hippocampal neurons and glial cells in the cerebral cortex of adult Wistar rats fed with different concentrations of iodine water for 3 and 6 months. To investigate the effects of iodine excess on cerebral cortex and hippocampal function in adult rats. Methods in January, 60 day old Wistar rats were randomly divided into six groups: low iodine group (Li group), and adequate iodine group (NI group). 10 times iodine group (10HI group). 50 times iodine group and 100 times iodine group 100 HI group. Li group were fed with diet (average iodine content was 50 渭 g / kg 路kg ~ (-1)), drinking deionized water; The NI group and the high iodine group were fed with normal diet (average iodine content was 300 渭 g / kg) for drinking tap water (iodine content was 5 渭 g / L). Different concentrations of Ki tap water were consumed in each high iodine group. The iodine intake of each high iodine group was 5 times that of NI group and 10 times as much as that of NI group according to the daily intake and quantity of drinking water. The weight of the whole brain and the morphological changes of pyramidal cells in the pyramidal cell layer, hippocampal neurons and glial cells in the cerebral cortex were observed after feeding for 3 and 6 months. The volume density of NSE positive neurons and GFAP positive glial cells was determined by immunohistochemical staining and silver plated staining. The mean gray value of cytoplasm and the number of basal dendrites of pyramidal cells in the pyramidal cell layer in cerebral cortex were analyzed by morphometrics. Results 1. There was no significant difference in the absolute and relative weight of the whole brain between the iodine excess group and the NI group in 3 and 6 months of the experiment, and the comparison between the six month Li group and the NI group in the same month. 2. The absolute weight of the whole brain decreased significantly, but there was no significant difference in the relative weight. 2. The results of hippocampal study: compared with NI group in 3 and 6 months. There was no significant difference in NSE positive neurons in hippocampal CA3 area, but the degree of cytoplasmic positive reaction was different. Compared with NI group in the same month, the 100HI group was compared with the NI group in the same month (P 0.01). Compared with NI group, the average gray value of NSE positive neurons in 50HI group was significantly lower than that in NI group. The mean gray value of NSE positive neurons in Li group was significantly lower than that in NI group in 6 months. Experimental 3 months each high iodine group, experiment 6 months Li group and each iodine excess group and the same month NI group
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R361

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