内质网应激对3T3-L1脂肪细胞成纤维细胞生长因子21 mRNA表达的调节作用

发布时间：2018-01-17 19:10

本文关键词：内质网应激对3T3-L1脂肪细胞成纤维细胞生长因子21 mRNA表达的调节作用　出处：《中国医药生物技术》2014年06期 　论文类型：期刊论文

【摘要】：目的在m RNA水平探讨内质网应激对3T3-L1脂肪细胞成纤维细胞生长因子21表达的影响。方法利用经典鸡尾酒法诱导3T3-L1前脂肪细胞分化,再用毒胡萝卜内酯分别按照剂量梯度(0、6.25、12.5、25、50和100 nmol/L)和时间梯度(0、1、3、6和12 h)进行处理,实时PCR检测内质网应激相关蛋白C/EBP同源蛋白和葡萄糖调节蛋白78以及成纤维细胞生长因子21 m RNA的表达水平。结果毒胡萝卜内酯能诱导分化成熟的3T3-L1脂肪细胞发生内质网应激,促进C/EBP同源蛋白、葡萄糖调节蛋白78和成纤维细胞生长因子21 m RNA水平显著升高,且与毒胡萝卜内酯的剂量和处理时间呈正相关。当用100 nmol/L毒胡萝卜内酯处理6 h后,成纤维细胞生长因子21 m RNA水平显著增高,为对照组的114.55倍(P0.05)。蛋白激酶C抑制剂钙磷酸蛋白C能阻断由毒胡萝卜内酯诱导的内质网应激,并伴随成纤维细胞生长因子21 m RNA水平的下降。结论内质网应激能上调3T3-L1脂肪细胞成纤维细胞生长因子21 m RNA表达,且可被蛋白激酶C抑制剂钙磷酸蛋白C阻断。
[Abstract]:Objective to investigate the level of RNA m in the endoplasmic reticulum stress effects of fibroblast growth factor 21 expression in 3T3-L1 adipocytes. Methods to induce the differentiation of 3T3-L1 preadipocytes using classic cocktail method, and thapsigargin respectively according to the dose gradient (0,6.25,12.5,25,50 and 100 nmol/L) and time gradient (0,1,3,6 and 12 h) for processing, real-time PCR detection endoplasmic reticulum stress related protein C/EBP protein and glucose regulated protein 78 and expression of fibroblast growth factor 21 m RNA. The thapsigargin induced endoplasmic reticulum stress in differentiated 3T3-L1 adipocytes, promote C/EBP homologous protein, glucose regulated protein 78 and fibroblast growth factor 21 m RNA level increased significantly and there, the dose and treatment time with thapsigargin. When the thapsigargin treatment with 100 nmol/L 6 h, a fine fiber Cell growth factor 21 m RNA levels were significantly higher, 114.55 times higher than that of control group (P0.05). Protein kinase C inhibitor Calphostin C can inhibit endoplasmic reticulum stress induced by thapsigargin, accompanied by the decrease of fibroblast growth factor 21 m RNA level. Conclusion the endoplasmic reticulum stress can upregulate 3T3-L1 adipocytes fibroblast growth factor 21 expression of M RNA, and can be a protein kinase C inhibitor Calphostin C blocking.

【作者单位】：温州医科大学药学院;武汉华大医学检验所有限公司;
【基金】：国家自然科学基金(81170813;81300311) 浙江省自然科学基金(Y2100048;LY12H03001;LQ13H280002) 浙江省重点科技创新团队项目(2010R50042)
【分类号】：R329.2
【正文快照】： 成纤维细胞生长因子(fibroblast growth factor,FGF)家族共有22个成员,分为7个亚家族,其主要功能涉及胚胎发育、细胞增殖、组织修复和肿瘤发生等[1-3]。2000年,Nishimura等[4]首次从小鼠胚胎中分离得到FGF21。研究发现,FGF21是FGF家族中的一个非典型性成员,其主要生物学功能是

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