反流性食管炎和抗胃食管反流手术动物模型的建立与实验研究

发布时间：2018-01-17 20:03

本文关键词：反流性食管炎和抗胃食管反流手术动物模型的建立与实验研究　出处：《河北医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 目的:通过建立反流性食管炎(Reflux Esophagitis,RE)动物模型及抗反流手术模型,观察食管大体形态和组织学改变,检测食管标本中血红素加氧酶(HO-1)、诱导型一氧化氮合酶(NOS)及热休克蛋白70(HSP70)水平,分析比较大鼠反流性食管炎中各反流因素的作用,同时为反流性食管炎尤其胃食管重建手术后吻合口反流的防治提供实验基础以及理论依据。方法:选用6周龄健康雄性SD大鼠120只(230~250g),随机分为6组,每组20只,建立反流性食管炎SD大鼠模型(单纯酸反流、单纯胆汁反流、混合反流各20只)、抗反流手术模型(食管胃套入吻合术动物模型20只)、单纯胃食管吻合动物模型及假手术动物模型各20只,观察各模型组动物存活情况、食管标本肉眼表现及食管标本病理组织学变化(HE染色),并应用逆转录聚合酶链反应法(RT-PCR技术)检测食管中血红素加氧酶(HO-1)、诱导型一氧化氮合酶(iNOS)及热休克蛋白(HSP70)的表达。利用统计软件SPSS10.0对数据进行统计学分析。结果 1单纯酸反流性食管炎动物模型的检测结果:①存活情况:术后第4、8、12、16周存活率分别为100%、95%、95%、95%。②食管粘膜肉眼表现分级:术后4周0级
[Abstract]:Objective: to observe the changes of gross morphology and histology of esophagus by establishing reflux esophagitis reflux model and anti-reflux operation model. The levels of heme oxygenase (HO-1), inducible nitric oxide synthase (NOS) and heat shock protein 70 (HSP70) were measured in esophageal specimens. To analyze and compare the effects of reflux factors in reflux esophagitis in rats, and to provide experimental basis and theoretical basis for the prevention and treatment of anastomotic reflux in rats with reflux esophagitis, especially after gastroesophageal reconstruction. Methods: 120 healthy male SD rats aged 6 weeks were randomly divided into 6 groups with 20 rats in each group to establish the SD rat model of reflux esophagitis (simple acid reflux). There were 20 bile reflux, 20 mixed reflux, 20 anti-reflux operation models (20 gastroesophageal anastomosis animal models, 20 simple gastroesophageal anastomosis animal models and 20 sham operation animal models). The survival of the animals in each model group, the naked appearance of esophageal specimens and the histopathological changes of esophageal specimens were observed by HE staining. The heme oxygenase (HO-1) in esophagus was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of inducible nitric oxide synthase (iNOS) and heat shock protein (HSP70) were analyzed by statistical software SPSS10.0. Results 1 the results of the animal model of simple acid reflux esophagitis were as follows: the survival rate at 1216 weeks after operation was 100%, 95% and 95%, respectively. 95. 2 grade of unaided esophageal mucosa: grade 0 4 weeks after operation
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R655.4;R-332

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