SCF和G-CSF联合动员小鼠外周血培养间充质干细胞的生物学特性的初步研究

发布时间：2018-01-21 20:14

本文关键词： 间充质干细胞干细胞因子粒细胞集落刺激因子外周血软骨细胞　出处：《河北医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 背景: 间充质干细胞(MSC)是指一群具有向成骨细胞、成软骨细胞、成脂肪细胞、肌腱细胞甚至肝脏细胞、神经细胞等多种细胞分化潜能的多能干细胞。由于其分化的多样性及在体外易分离培养和扩增的特性使其成为组织工程学的理想种子细胞。在骨科领域具有广泛的应用前景,主要应用于骨修复和骨形成、软骨修复、肌腱和韧带组织修复等。MSC主要存在于骨髓、脂肪组织、软骨及骨组织、脐带血、胎盘中,其中以骨髓中含量最为丰富。外周血中是否存在MSC仍有很大争议。本试验通过联合应用干细胞因子(SCF)和粒细胞集落刺激因子(G-CSF)动员小鼠外周血培养MSC,探索外周血MSC的培养方法和生物学特性。目的: 探讨联合利用SCF和G-CSF动员小鼠外周血培养MSC的分离、培养和扩增方法。并初步研究在体外单层培养条件下向软骨细胞方向诱导的条件,为临床上利用外周血来源的间充质干细胞修复关节软骨缺损提供前期的资料和方法。方法: 将40只4-6周龄BALB/C小鼠随机分为两组。试验组应用SCF及G-CSF动员后的小鼠外周血,2只BALB/C小鼠外周血为一份,对照组应用注射生理盐水小鼠的外周血作
[Abstract]:Background: Mesenchymal stem cell (MSCs) is a group of osteoblasts, chondroblasts, adipoblasts, tendon cells and even liver cells. Pluripotent stem cells with differentiation potential such as nerve cells. Due to their diversity of differentiation and easy isolation, culture and expansion in vitro, they are ideal seed cells for tissue engineering. They are widely used in orthopaedics. The prospect of its application. MSC mainly exists in bone marrow, adipose tissue, cartilage and bone tissue, umbilical cord blood and placenta. The presence of MSC in peripheral blood is still controversial. In this study, the combination of stem cell factor (SCF) and granulocyte colony stimulating factor (G-CSF) was used. MSC was mobilized in peripheral blood of mice. Objective: to explore the culture methods and biological characteristics of peripheral blood MSC. To explore the method of isolation, culture and amplification of MSC from peripheral blood of mice mobilized by SCF and G-CSF, and to study the conditions of inducing MSC to chondrocytes under monolayer culture in vitro. To provide clinical data and methods for repairing articular cartilage defects with peripheral blood derived mesenchymal stem cells. Methods: Forty 4-6 week-old BALB/C mice were randomly divided into two groups. In the experimental group, the peripheral blood of 2 BALB/C mice mobilized by SCF and G-CSF was one. The control group was treated with peripheral blood of mice injected with normal saline.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

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