SD大鼠数字解剖数据集研究

发布时间：2018-01-22 06:44

本文关键词： 断层解剖数据集数字解剖数据集三维解剖结构模型图像配准图像分割三维重建　出处：《华中科技大学》2006年博士论文　论文类型：学位论文

【摘要】： 如今实验动物已经成为遗传学、疾病以及药物开发等研究领域最基本的研究工具之一。为了方便各种生物学信息的共享和整合,同时出于对动物生存权利及动物福利的考虑,小动物的结构与功能信息的数字化已经成为国内外各研究机构的研究热点。数字解剖数据集以其更加准确的、直观的、图形化表示方法等具有广泛应用价值的特点,引起了越来越多的科研人员的关注。本文通过对不同断层解剖数据获取方式分析、对比,基于冰冻断层技术搭建了适于小动物研究的断层解剖成像系统。以SD大鼠为例,构建了SD大鼠断层解剖数据集,对断层解剖数据集的构建方法、工艺路线、技术参数选择、可能出现的问题及其解决方法进行了系统的研究。并且基于SD大鼠断层解剖数据集,构建了SD大鼠数字解剖数据集,并对其技术方案和构建技术进行了深入的研究。课题主要实验结果和结论如下所示: (1)基于冰冻铣削技术搭建了适于小动物研究的断层解剖成像系统,技术参数为:断层切削设备的最小进给量为0.001 mm,重复定位精度为0.01/300 mm。该系统具有如下特点,大体积样品成像;空间分辨率高;侵入型成像方法;断层图像具有生物样品原有的组织纹理、颜色等细节信息。该系统可以获得小动物的精确结构信息,为小动物研究提供了一种新的、有效的手段。 (2)本文以两只成年SD大鼠为例,叙述了断层解剖成像的实验过程。并且基于适于小动物研究的断层解剖成像系统,获取了SD大鼠断层解剖数据集(体素尺寸为20μm×20μm×20μm,图像分辨率为4,600×2,580×24 bit,BMP格式,共9475张,314.68 GB)和血管灌注的SD大鼠断层解剖数据集(体素尺寸为20μm×20μm×100μm,图像分辨率为4,917×3,446×24 bit,BMP格式,共1646张,77.9 GB)。 (3)探讨了构建数字解剖数据集的技术方案和构建技术,并且基于SD大鼠断层解剖数据集和血管灌注的SD大鼠断层解剖数据集,构建了SD大鼠数字解剖数据集,获得了高精度的SD大鼠三维解剖结构模型及动脉系统结构模型,具有广泛的教育及应用价值。 (4)“Visual Rat Atlas”和“Visual Rat Explorer”分别显示SD大鼠的不同截面的系列断层图像、解剖图谱和SD大鼠的三维解剖结构模型。软件的使用使得研究人员能够方便的对数字解剖数据集进行分析和计算,是一种方便实用的研究工具。研究证明适于小动物研究的断层解剖成像系统,为小动物结构信息研究提供了一种有效的手段。基于该系统构建的数字解剖数据集可以作为一个框架整合更多生物学信息,研究不同层次、不同水平的生物学信息,为小动物研究及其在生物医学研究中提供了应用模型。
[Abstract]:Nowadays , experimental animals have become one of the most basic research tools in genetics , disease and drug development . In order to facilitate the sharing and integration of various biological information , the digitization of the structure and function information of small animals has become a hot topic at home and abroad in order to facilitate the sharing and integration of animal survival rights and animal welfare . In this paper , a tomographic imaging system suitable for small animals has been established by means of analysis and comparison of different anatomical data . Based on SD rats , an anatomical data set of SD rats was constructed . The data set of SD rats was constructed on the basis of SD rats , and its technical scheme and construction techniques were studied . ( 1 ) Based on the frozen milling technology , a tomographic imaging system suitable for small animal studies is constructed . The technical parameters are as follows : the minimum feeding amount of the fault cutting equipment is 0.001 mm , and the repeated positioning accuracy is 0.01 / 300 mm . The system has the following characteristics : large volume sample imaging ; high spatial resolution ; invasive imaging method ; and the system can obtain accurate structural information of small animals , and provides a new and effective means for the study of small animals . ( 2 ) In this paper , two adult SD rats were used as an example to describe the experimental procedure of tomographic imaging . Based on a tomographic imaging system suitable for small animal studies , SD rats were acquired ( voxel size 20 渭m 脳 20 渭m 脳 20 渭m , image resolution 4 , 600 脳 2 , 580 脳 24 bit , BMP format , total 9475 , 314.68 GB ) and vascular perfusion SD rats . The image resolution was 4 , 917 脳 3 , 446 脳 24 bit , BMP format ( 1646 , 77.9 GB ) . ( 3 ) The technical scheme and the construction technology of constructing the digital anatomic data set were discussed , and the SD rat digital anatomical data set was constructed based on the SD rat tomographic dataset and the vascular perfusion SD rat , and the model of the three - dimensional anatomical structure and the system structure model of SD rats were obtained , which had extensive education and application value . ( 4 ) " Visual Rat Atlas " and " Visual Rat Explorer " respectively show the series of tomographic images , anatomical maps and three - dimensional anatomical structure models of SD rats . The use of the software enables the researchers to analyze and calculate the digital anatomical data sets conveniently , and is a convenient and practical research tool . The research proves that the tomographic imaging system suitable for small animal studies provides an effective means for the study of small animal structure information . The digital anatomical data set constructed on the basis of the system can be used as a framework to integrate more biological information , study the biological information at different levels and levels , and provide an application model for the research of small animals and in biomedical research .

【学位授予单位】：华中科技大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R-332

【引证文献】