成都地区部分人群军团菌抗体调查及LpHSP60基因克隆与表达

发布时间：2018-01-29 21:23

本文关键词： 军团菌感染抗体效价微量凝集试验嗜肺军团菌热休克蛋白60 克隆表达　出处：《四川大学》2005年硕士论文　论文类型：学位论文

【摘要】：军团菌病是一种以肺炎为主要表现的急性细菌性传染病,是非典型肺炎的重要组成部分,严重危害人类健康。世界上已有多个国家和地区相继报道发现该病。许多国家已将军团菌病列为法定传染病。作为其病原体的军团菌是兼性胞内寄生菌,普遍存在于天然水、人工水环境或在原生动物胞内寄居。军团菌是引起社区获得性肺炎和医院感染的常见病原体。随着空调、加湿器在现代生活中的普及,军团菌感染会大量增加,危害会更严重。目前已知军团菌属有42个种,64个血清型,其中嗜肺军团菌(Legionella pneumophila, Lp)是军团菌病的主要病原菌。人类主要通过吸入含有军团菌的气溶胶而感染。军团菌侵入肺泡巨噬细胞后,能抑制吞噬体—溶酶体的融合,并在其中繁殖,引起以非典型肺炎和Pontiac热为主的疾病,若治疗不当,病死率极高。流行病学调查结果表明我国人群中存在不同程度和不同种型的军团菌感染。我国已将军团菌病列为需要重点防治的一种新发传染病,但目前尚无理想的预防措施。细胞介导的免疫反应在宿主抵御军团菌中起着重要的作用,而体液免疫在抵御疾病中的作用较小。研究表明,热休克蛋白(Heat Shock Protein, HSP)对多数病原微生物来说是一种免疫保护性抗原。嗜肺军团菌热休克蛋白60(LpHSP60)是细胞内嗜肺军团菌合成最丰富的蛋白质之一,主要在细菌表面表达。有研究发现嗜肺
[Abstract]:Legionella disease is an acute bacterial infectious disease characterized by pneumonia, which is an important component of atypical pneumonia. The disease has been reported in many countries and regions in the world. Legionella has been listed as a legal infectious disease in many countries. Legionella, as its pathogen, is a facultative intracellular parasite. Legionella is a common pathogen of community-acquired pneumonia and nosocomial infection. With the popularization of air conditioning humidifier in modern life. Legionella pneumophila is known to have 42 species and 64 serotypes of Legionella. Lpis is the main pathogen of Legionella. Human is infected by inhalation of aerosol containing Legionella. Legionella can inhibit the fusion of phagocytosome and lysosome after invading alveolar macrophages. And in which reproduction, leading to atypical pneumonia and Pontiac fever disease, if not properly treated. The result of epidemiological investigation shows that Legionella infection exists in different degree and different types in Chinese population. Legionella has been listed as a new infectious disease which needs to be prevented and controlled in our country. However, there is no ideal preventive measures. Cellular mediated immune response plays an important role in host resistance to Legionella, while humoral immunity plays a less important role in disease resistance. Heat shock protein Shock Protein. Heat shock protein (HSP60) is one of the most abundant proteins synthesized by Legionella pneumophila. Mainly expressed on the surface of bacteria.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392

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