人芽囊原虫形态学和致病性的研究

发布时间：2018-01-29 21:28

  本文关键词： 人芽囊原虫 体外培养 形态学 动物模型 病理变化　出处：《郑州大学》2006年硕士论文　论文类型：学位论文

【摘要】：背景和目的 人芽囊原虫(Blastocystis hominis, B. hominis)是一种越来越引起学者们关注的肠道寄生性原虫。自1911由Alexief首次发现以来，由于B. hominis具有形态多变及光镜下不宜辨认等特点，一直未引起学术界的普遍重视。我国在B. hominis研究方面起步较晚，1990年以来虽然少数学者曾进行了形态学、病理学、区域流行病学方面的研究，但各研究仍属起步阶段。对B. hominis进一步实验研究很有必要。 本研究结合B. hominis独特的生化待点和可能的生活周期，用多种普通和特殊的染色方法对B. hominis的形态进行了光镜、电镜的观察，以进一步了解其形态特点，并结合形态，对B. hominis某些细胞器功能进行了初步探讨。然后将培养的不同数量的空泡型B. hominis感染免疫功能正常和免疫功能低下小鼠，比较不同数量感染的差异，，免疫功能正常及免疫功能低下小鼠感染B. hominis的差异。小鼠感染实验及建立B. hominis的动物感染模型是为研究B. hominis的致病性及致病机理奠定了基础。 材料和方法 1．形态学观察 本实验用B. hominis阳性的粪便标本接种于加有10％灭活胎牛血清的locke氏-鸡蛋双相培养基(LES)和1640培养基(RPMI-1640)培养，37℃分离培养，48-72h转种一次传代保种，转种时加入青霉素和链霉素。转种后每日吸取培养液经碘液染色和H．E染色，光镜下观察虫体各期形态并拍照。同时将培养液制备成电镜标本，置扫描电镜下观察各期虫体的超微形态结构。 2．B. hominis感染小鼠
[Abstract]:Background and purpose Blastocystis hominis. B. hominis is an intestinal parasitic protozoa that has attracted increasing attention from researchers since it was first discovered by Alexief in 1911. Because of its characteristics of changeable morphology and unsuitable recognition under light microscope, B. hominis has not been paid much attention to in academic circles. Our country started late in the research of B. hominis. Since 1990, a few scholars have studied morphology, pathology, and regional epidemiology. However, the studies are still at the initial stage. It is necessary to further study the B. Hominis experiment. In this study, the morphology of B. hominis was examined by various ordinary and special staining methods combined with the unique biochemistry of B. hominis and its possible life cycle. Electron microscope observation to further understand its morphological characteristics, and combined with morphology. The function of some organelles of B. hominis was preliminarily studied. Then the vacuolar type B. Hominis was infected with normal immune function and hypoimmune function in mice. To compare the difference of infection quantity. The infection of B. hominis in mice with normal immune function and hypoimmune function was different. The infection test of mice and the establishment of animal infection model of B. hominis were used to study the infection of B. hominis in mice. The pathogenicity and pathogenesis of hominis lay the foundation. Materials and methods 1. Morphological observation In this experiment, B.#en0# positive fecal specimens were inoculated on locke's and egg biphasic medium with 10% inactivated fetal bovine serum. RPMI-1640). The seeds were separated and cultured at 37 鈩

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