JWA在K562和MCF-7细胞应答氧化应激中的作用及其分子机制

发布时间：2018-01-31 03:28

本文关键词： JWA K562细胞 MCF-7细胞氧化应激热休克蛋白质热休克因子 p53 procaspase-3　出处：《南京医科大学》2005年硕士论文　论文类型：学位论文

【摘要】：人类许多疾病与接触的环境因素有关,各种物理的、化学的和生物的有害因素暴露后,机体细胞很快产生应答,表现为一系列基因有序地发生表达水平的变化,使细胞尽快适应变化的环境而维持内平衡。有害环境因素可直接引起细胞基因组的损伤,或化学物在体内特异性分布和代谢后损伤基因组结构,或干扰细胞内的信号转导通路,最后引起疾病。 JWA基因是周建伟等1998年发现并克隆的受维甲酸诱导的新基因,并获得美国基因库新基因编号(AF070523.1)。通过对其结构分析发现,JWA基因启动子序列中含有包括热休克反应元件(hRE)、应激反应元件(SRE)等多种反应元件。近几年本课题组研究表明,JWA是一种新的微管相关蛋白,不仅广泛地参与调节多种分化诱导剂(如TPA、ATRA、4HPR和AS_2O_3)涉及的信号通路,而且活跃地参与细胞对应激刺激(如氧化应激和热应激)的应答。但是JWA基因是如何参与调控细胞氧化应激的信号通路尚不清楚。本课题用不同浓度的过氧化氢(H_2O_2)处理K562(慢性骨髓性
[Abstract]:Many human diseases are related to environmental factors in contact. After exposure to various physical, chemical and biological harmful factors, the cells of the body quickly respond, which is manifested by a series of gene expression changes in an orderly manner. The harmful environmental factors can directly cause the damage of the cell genome, or the specific distribution of chemicals in the body and damage to the genome structure after metabolism. Or interfere with the intracellular signal transduction pathway, and finally cause disease. JWA gene is a novel gene induced by retinoic acid found and cloned by Zhou Jianwei in 1998. The new gene number AF070523.1in American gene bank was obtained. It was found that the promoter sequence of JWA gene contained heat shock response element (hREE). In recent years, our research has shown that JWA is a new microtubule-associated protein, which is not only involved in the regulation of various differentiation inducers (such as TPA). ATRA-4HPR and ASSP _ 2o _ 3) are involved in the signal pathway. But it is not clear how JWA gene is involved in the regulation of cellular oxidative stress signaling pathway. In this study, K562 (chronic bone marrow) was treated with different concentrations of H _ 2O _ 2 hydrogen peroxide.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

【共引文献】