小鼠胚胎心流出道近段心室化形成右心室小梁部

发布时间：2018-02-02 16:54

本文关键词： 胚胎心流出道 GATA- α-横纹肌肌动蛋白 α-平滑肌肌动蛋白增殖细胞核抗原免疫组织化学小鼠　出处：《解剖学报》2014年05期 　论文类型：期刊论文

【摘要】：目的探讨小鼠胚胎心流出道快速缩短及右心室形成机制。方法用抗α-横纹肌肌动蛋白(SCA)、抗肌球蛋白重链(MHC)抗体标记心肌,抗GATA-4抗体标记心肌及其前体细胞,抗α-平滑肌肌动蛋白(SMA)抗体标记早期心肌细胞,抗增殖细胞核抗原(PCNA)抗体显示增殖细胞,抗人/鼠活性Caspase-3(CAS-3)抗体检测凋亡早期细胞,对胚龄9d(E9)~E12(不同胚龄胚胎各取3~5只)小鼠胚胎心连续切片行免疫组织化学染色。结果E11时动脉囊及流出道远端心肌界退却至心包腔内,GATA-4、SCA、SMA染色示第二生心区前体细胞不断分化为心肌细胞添加在心动脉端使流出道延长。小鼠胚胎心流出道于E12缩短,缩短前及缩短过程中全长未检测到CAS-3阳性细胞。E10~12时右心室及流出道近段心肌不断增生形成小梁并侵入邻近的流出道嵴内,流出道近端嵴逐渐小梁状心肌化改建为右心室壁;E12时近段间充质性流出道嵴内出现散在的SCA、SMA阳性心肌细胞及与流出道心肌相延续的SCA、SMA弱阳性心肌细胞流,这些结果表明近段流出道心肌小梁化、流出道嵴小梁状肌化形成了右心室小梁部。结论小鼠胚胎流出道近段心室化致右心室小梁部形成及流出道快速缩短,心肌细胞凋亡及转分化对流出道快速缩短的作用甚微。
[Abstract]:Objective to investigate the mechanism of rapid shortening of the outflow tract and the formation of right ventricle in mouse embryo. Methods Myocardium was labeled with anti-伪 -rhabdomysmal actin (SCA) and anti-myosin heavy chain (MHC) antibody. Anti GATA-4 antibody labeled myocardium and its precursor cells, anti 伪 smooth muscle actin antibody labeled early myocardial cells, anti proliferating cell nuclear antigen (PCNA A) antibody showed proliferating cells. The anti-human / mouse active Caspase-3 CAS-3) antibody was used to detect the early apoptotic cells. The embryo age was 9 days old, E9 and E12 (5 embryos were taken from each embryo at different ages). Results the myocardial boundary of the arterial sac and the distal outflow tract retreated to the pericardial cavity at E11. GATA-4 SCA- SMA staining showed that the secondary cardiac progenitor cells were continuously differentiated into cardiomyocytes and the outflow tract was prolonged at the end of the cardiac artery. The outflow tract of mouse embryo heart was shortened at E12. CAS-3 positive cells were not detected in the whole length before and during the shortening. The right ventricle and the proximal segment of the outflow tract proliferated to form trabeculae and infiltrate into the adjacent outflow tract crest from the right ventricle and the proximal outflow tract. The proximal crest of outflow tract was gradually remodeled into right ventricular wall by trabecular-shaped myocardium. At E12, there were scattered SCA- SMA positive cardiomyocytes in the mesenchymal outflow tract crest and SCA- SMA weakly positive cardiomyocytes flowing along with the outflow tract myocardium. These results suggest that trabecularization of the proximal outflow tract and trabecularization of the outflow tract crest form the right ventricular trabeculae. Conclusion the formation of the right ventricular trabeculae and the rapid shortening of the outflow tract are caused by the proximal ventricular outflow tract of mouse embryo. Cardiomyocyte apoptosis and transdifferentiation have little effect on rapid shortening of convection exit.
【作者单位】：山西医科大学组织学与胚胎学教研室;
【基金】：国家自然科学基金资助项目(30771141,31200899) 山西省回国留学人员科研基金资助项目(2008-47) 山西省自然科学基金资助项目(2006011108)
【分类号】：R321
【正文快照】： 胚胎心流出道是原始心室和动脉囊间的一管状结构[1~3],其远端心肌延伸至心包折返处,近端止于球室曲处[4,5]。随着发育,右心室出现,流出道长度不断增加,其内出现两条纵行的流出道嵴。此时,流出道远端界为其远端心肌处,近端界为右心室小梁心肌起始处[2,5]或其嵴的近端。流出道重

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