基于光电检测与信息处理技术的纳米金免疫层析试条定量测试的研究

发布时间：2018-02-03 23:32

本文关键词： 纳米金免疫层析试条光电检测小波变换模糊聚类识别定量测试　出处：《福州大学》2005年博士论文　论文类型：学位论文

【摘要】：免疫测定是基础研究和临床检测中应用广泛、分析敏感的一种技术,遍及医学检验的各个领域。随着免疫测定技术的日新月异,许多新技术取代传统的实验方法,纳米金免疫层析试条就是其中一种。纳米金免疫层析试条具有检测效率高、方法简便、一步完成、无污染、试剂稳定、适用于单人份测定等特点,已受到临床检验界的极大关注,并发展成为临床检验的前沿领域。但是,目前纳米金免疫层析试条主要用于定性或半定量的快速免疫检测方法中,使试条的临床应用范围受到了限制。因此,纳米金免疫层析试条定量测试的研究具有重要的理论意义与应用价值。本文从理论上对纳米金免疫层析试条定量测试机理进行研究,以试条光谱峰特性曲线为研究重点,将纳米金免疫层析法与以光纤传感为核心的光电检测技术以及现代信息处理技术相结合,提出了纳米金免疫层析试条智能定量测试的新概念、新思路,从而实现了对纳米金免疫层析试条准确地定量测试,为临床免疫测定提供一种新的、有效的检测手段。本文主要进行以下研究工作: (1) 探讨纳米金免疫层析试条定量测试机理和光谱峰信号的基本特点,深入分析对定量测试造成影响的各种干扰因素,如生化噪声、光噪声、电噪声等,建立理想光谱峰曲线的数学表达式,研究适合纳米金免疫层析试条智能定量测试的新机理。 (2) 将小波变换、模糊聚类识别和小波神经网络等技术引入纳米金免疫层析试条定量检测中,完成对试条边缘特征提取、噪声信号滤除和曲线拟合等,使现代信息处理技术对纳米金免疫层析试条信号的分析从理论研究走向实际应用。本文提出基于现代信息处理技术的纳米金免疫层析试条智能定量测试新方法,拓展了现代信息处理技术在生物医学领域的应用。 (3) 根据朗伯—比尔定律和互补光原理,利用光电检测系统将纳米金免
[Abstract]:Immunoassay is widely used in basic research and clinical detection. It is a sensitive analytical technique, which is widely used in various fields of medical testing. With the rapid development of immunoassay technology. Many new technologies replace the traditional experimental methods, one of which is the nanocrystalline gold immunochromatographic strip. The nanocrystalline gold immunochromatographic strip has the characteristics of high detection efficiency, simple method, one-step completion, no pollution, stable reagent, suitable for single determination, and has been paid great attention to by the clinical laboratory. And has developed into the frontier field of clinical laboratory. However, at present, nanocrystalline gold immunochromatographic strips are mainly used in qualitative or semi-quantitative rapid immunoassay, which limits the clinical application of the strips. The study of gold nanoparticles immunochromatographic strip quantitative test has important theoretical significance and application value. In this paper, the mechanism of quantitative measurement of nanocrystalline gold immunochromatographic strip was studied theoretically, with the focus on the characteristic curve of the spectral peak of the strip. Combining nano-gold immunochromatography with optoelectronic detection technology based on optical fiber sensor and modern information processing technology, a new concept and new idea of intelligent quantitative test of nano-gold immunochromatographic strip are put forward. Thus, the gold nanoparticles immunochromatographic strip is tested accurately and quantitatively, which provides a new and effective method for clinical immunoassay. The main work of this paper is as follows: 1) to discuss the mechanism of quantitative measurement and the basic characteristics of spectral peak signal in nanoscale gold immunochromatographic strip, and to analyze in depth various interference factors, such as biochemical noise, light noise, electric noise and so on. The mathematical expression of the ideal spectral peak curve was established to study the new mechanism suitable for intelligent quantitative testing of nanocrystalline gold immunochromatographic strips. 2) the wavelet transform, fuzzy clustering recognition and wavelet neural network are introduced into the quantitative detection of the strip of nanoscale gold immunochromatography. The edge feature extraction, noise signal filtering and curve fitting are completed. This paper presents a new intelligent quantitative test method based on modern information processing technology to analyze the signal of nano-gold immunochromatographic strip from theoretical research to practical application. The application of modern information processing technology in biomedical field is expanded. According to Lambert-Beer 's law and complementary light principle, nanocrystalline gold can be eliminated by photoelectric detection system.
【学位授予单位】：福州大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392

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