基于小鼠肿瘤模型的建立及抗肿瘤药物的药效评价

发布时间：2018-02-24 00:30

本文关键词： 联合基因紫杉醇紫杉醇衍生物阿霉素细胞凋亡　出处：《首都师范大学》2006年硕士论文　论文类型：学位论文

【摘要】：恶性肿瘤严重威胁着人类的生存质量，被称为人类健康的第一杀手。近年来对恶性肿瘤的治疗虽取得了长足的进步，但临床用药仍然停留于文献资料和临床经验基础上，对不同肿瘤患者使用固定的治疗方案，不可避免地带来用药的盲目性及药物的耐药性，导致低疗效甚至无效，因此，治疗方案的选择是治疗成败的关键，而有效的治疗方案的确定取决于对肿瘤敏感性药物的选择和应用。因此寻找一种好的确定肿瘤药物敏感性的实验方法是当今研究的热点。本实验目的是建立在细胞水平和动物水平上建立药物评价模型，对几种抗肿瘤药物进行药效评价。细胞水平以人喉癌Hep-2细胞为靶细胞，，应用形态学观察、MTT分析法、DNA梯状电泳和流式细胞仪检测术(FCM)分别研究抗肿瘤药物(双基因p53／p21、紫杉醇和紫杉醇衍生物、阿霉素和阿霉素缓释微球)对人喉癌Hep-2细胞的细胞毒效应。结果发现，形态学观察细胞皱缩，核染色质凝聚或者断裂；MTT实验显示药物抑制Hep-2细胞增殖，并且存在着时间依赖和一定范围内的剂量依赖关系。三种抗肿瘤药物均能抑制人喉癌细胞Hep-2增殖的作用，这种作用与剂量、时间呈正相关。对人喉癌Hep-2细胞的细胞毒作用主要是通过诱导凋亡实现的。动物水平上以小鼠肉瘤S-180为模型，以肿瘤体积，抑瘤率为评价指标，评价三种抗肿瘤药物对实体瘤的抑制作用。研究结果发现三种药物对实体瘤具有明显的抑制作用，
[Abstract]:Malignant tumor is a serious threat to the quality of human life and is called the first killer of human health. Although great progress has been made in the treatment of malignant tumors in recent years, the clinical use of drugs remains on the basis of literature and clinical experience. The use of fixed treatment for different tumor patients will inevitably lead to blindness and drug resistance, resulting in low or even ineffective therapeutic effect. Therefore, the choice of treatment plan is the key to the success or failure of the treatment. The determination of effective treatment scheme depends on the choice and application of sensitive drugs to tumor. Therefore, it is a hot research topic to find a good experimental method to determine the sensitivity of tumor drugs. Establishment of drug evaluation models at cellular and animal levels, The efficacy of several antitumor drugs was evaluated. Human laryngeal carcinoma Hep-2 cells were used as target cells at the cell level. The antitumor drugs (double gene p53 / p21, paclitaxel and taxol derivatives) were studied by morphological observation and flow cytometry (FCM). The cytotoxic effects of adriamycin and adriamycin microspheres on human laryngeal carcinoma Hep-2 cells were observed. And there is a time dependence and a range of dose-dependent relationships. All three antitumor drugs could inhibit the proliferation of human laryngeal carcinoma cell line Hep-2, which was positively correlated with the dose and time. The cytotoxic effect on human laryngeal carcinoma Hep-2 cells was mainly achieved by inducing apoptosis. At the animal level, the mice sarcoma S-180 was used as the model, the tumor volume and the tumor inhibition rate were taken as the evaluation indexes to evaluate the inhibitory effect of three kinds of antitumor drugs on the solid tumor. The results showed that the three drugs had obvious inhibitory effect on the solid tumor.
【学位授予单位】：首都师范大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R96

【相似文献】