葡萄糖对ICR小鼠胚胎体外发育的影响

发布时间：2018-02-26 07:26

  本文关键词： 葡萄糖 小鼠 -细胞阻滞 胚胎培养 体外发育 囊胚率 桑椹胚 体外培养 孵化率 无糖　出处：《动物学研究》2007年05期 　论文类型：期刊论文

【摘要】：研究葡萄糖在小鼠早期胚胎体外发育中的作用。实验1将6—8周龄的ICR雌鼠超数排卵后与公鼠交配,收集1-细胞放入含0(对照组)、0.5、1、3、5、10mmol/L葡萄糖的CZB中培养;实验2将从超排的ICR雌鼠输卵管内收集的1-细胞放入无糖CZB中培养,分别于1细胞、2细胞、4细胞、桑椹胚阶段移入含3.0mmol/L葡萄糖(最适浓度)的CZB中,培养24h后又移回到无糖CZB中(桑椹胚阶段除外)继续培养以及整个胚胎培养过程均在含糖CZB中,对照组胚胎培养全程均在无糖CZB中。每组胚胎于37℃、5%CO2培养箱中培养120h,每24h在倒置显微镜下观察胚胎发育情况,分别计算2-细胞率、4-细胞率、桑椹胚率、囊胚率和孵化率,并进行囊胚细胞计数。结果显示,小鼠胚胎在含糖CZB中与在无糖CZB中4-细胞发育率无差异;含糖CZB中囊胚率显著高于对照组;3.0mmol/L浓度组囊胚细胞数显著高于其余组;2-细胞至4-细胞、4-细胞至桑椹胚前添加葡萄糖囊胚率显著高于对照组,1-细胞至2-细胞、桑椹胚及其以后阶段添加葡萄糖囊胚率与对照组无差异。实验证实,在ICR小鼠胚胎体外培养中加入葡萄糖不会导致2-细胞阻滞;葡萄糖浓度增至10mmol/L对ICR小鼠胚胎无毒性作用;ICR小鼠胚胎体外培养的最适葡萄糖浓度为3.0mmol/L;2-细胞至4-细胞、4-细胞至桑椹胚前添加葡萄糖是必要的。
[Abstract]:To study the role of glucose in the development of mouse early embryos in vitro. In experiment 1, ICR female mice aged 6-8 weeks were superovulated and mated with male mice, and 1- cells were collected and cultured in CZB containing 0 (control group) (control group, 0. 5 ~ 0. 5) and 10 mmol / L glucose. In experiment 2, 1- cells collected from oviducts of superovulation ICR female oviducts were cultured in sugar-free CZB and transferred into CZB containing 3.0 mmol / L glucose (optimal concentration) at morulae stage. After 24 hours of culture, it was transferred back to sugar-free CZB (except morula stage) and the whole process of embryo culture was in sugary CZB. The embryos of the control group were cultured in sugar-free CZB for 120 hours in 37 鈩，

本文编号：1537050