电离辐射对小鼠巨噬细胞Toll信号传导通路的影响

发布时间：2018-02-26 07:26

本文关键词： 巨噬细胞 TLR4 CD14 MyD88 IL-18　出处：《吉林大学》2005年硕士论文　论文类型：学位论文

【摘要】：免疫细胞间或细胞与介质间相互识别的物质基础是免疫细胞膜分子,包括细胞表面的多种抗原、受体和其它分子。研究电离辐射对免疫细胞膜分子的作用,有助于深入了解辐射免疫效应的本质,为临床应用放射治疗脊柱恶性肿瘤提供相应的理论依据。本研究在以往研究的辐射免疫效应机制基础上,进一步探讨电离辐射全身照射后,小鼠腹腔巨噬细胞TLRs 介导信号传导通路的机制。不同剂量全身照射小鼠后,采用流式细胞术检测小鼠腹腔巨噬细胞Toll 信号通路上跨膜受体CD14、TLR4 以及细胞内转接蛋白MyD88 表达的变化,采用ELISA 法检测效应产物IL-18表达的变化。结果显示高低剂量X 射线全身照射小鼠后,均能增强该信号通路上的跨膜受体、转接蛋白以及效应产物的表达。说明电离辐射效应影响小鼠机体免疫应答,并且导致腹腔巨噬细胞的免疫因子IL-18 分泌增高,这是通过腹腔巨噬细胞固有免疫Toll信号传导通路,即细胞膜上的受体CD14、TLR4/TLRs→胞内转接蛋白MyD88→细胞核转录因子NF-κB→效应因子IL-18 来完成的。
[Abstract]:The material basis for mutual recognition between immune cells or between cells and mediators is the immune cell membrane molecules, including multiple antigens, receptors and other molecules on the surface of the cell. It is helpful to understand the nature of radio-immune effect and to provide theoretical basis for clinical radiotherapy of spinal malignant tumors. In this study, the mechanism of TLRs mediated signal transduction by peritoneal macrophages in mice was further studied on the basis of the previous studies on the mechanism of radiation immunity. Flow cytometry was used to detect the expression of transmembrane receptor CD14TLR4 and MyD88 on the Toll signaling pathway of mouse peritoneal macrophages. ELISA method was used to detect the expression of IL-18. The results showed that the transmembrane receptors in the signaling pathway were enhanced in mice exposed to high and low doses of X-rays. The expression of transfer protein and its effector products. The results show that ionizing radiation effects the immune response of mice and leads to the increase of IL-18 secretion of peritoneal macrophages, which is through the intrinsic immune Toll signal transduction pathway of peritoneal macrophages. The receptor on the cell membrane, CD14, TLR4 / TLRs. 鈫扞ntracellular transfer protein MyD88. 鈫扤uclear transcription factor NF- 魏 B. 鈫扙ffect factor IL-18 to complete.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392

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