同型半胱氨酸对肝细胞增殖及CyclinD1、ALT和AST表达的影响

发布时间：2018-02-26 14:27

本文关键词： 肝细胞细胞增殖同型半胱氨酸细胞周期素D 丙氨酸转氨酶天冬氨酸氨基转移酶　出处：《重庆医学》2014年12期 　论文类型：期刊论文

【摘要】：目的探讨同型半胱氨酸(Hcy)对肝细胞增殖及细胞周期素D1(CyclinD1)、丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)表达的影响。方法将HL-7702细胞体外常规培养,分别用0、50、100、200、500μmol/L Hcy刺激细胞,分别于刺激后6、12、24h采用MTT法检测肝细胞的增殖情况;采用荧光定量PCR(RT-qPCR)检测CyclinD1mRNA的表达;采用微板法检测各组细胞培养液中ALT和AST的变化。结果不同浓度Hcy刺激肝细胞不同时间后,细胞的增殖受到抑制,其中100、200、500μmol/L Hcy组与对照组比较差异有统计学意义(P0.01),24h作用最明显;不同浓度Hcy刺激肝细胞24h后,CyclinD1mRNA的表达显著下降(P0.01),细胞培养液中ALT和AST水平显著升高(P0.01)。结论 Hcy可以抑制肝细胞增殖,并引起CyclinD1的mRNA表达下降,ALT和AST释放增多。
[Abstract]:Objective to investigate the effects of homocysteine (HCH) on the proliferation of hepatocytes and the expression of cyclin D 1 cyclin D 1, alanine aminotransferase (alt) and aspartate aminotransferase (AST). Methods the HL-7702 cells were cultured in vitro and stimulated with #number0# 渭 mol/L Hcy. The proliferation of hepatocytes was detected by MTT assay, the expression of CyclinD1mRNA was detected by fluorescence quantitative PCR RT-qPCR, and the changes of ALT and AST in the culture medium of each group were detected by microplate method. Results different concentrations of Hcy stimulated hepatocytes for different time. The proliferation of the cells was inhibited, especially in the 100,200,500 渭 mol/L Hcy group compared with the control group. There was a significant difference between the 100,200,500 渭 mol/L Hcy group and the control group. The expression of cyclin D1 mRNA in hepatocytes was significantly decreased after stimulated with different concentrations of Hcy for 24 hours, and the levels of ALT and AST in cell culture medium were significantly increased. Conclusion Hcy can inhibit the proliferation of hepatocytes and induce the decrease of mRNA expression of CyclinD1 and increase the release of alt and AST.
【作者单位】：宁夏医科大学检验学院;宁夏医科大学基础学院病理生理教研室;宁夏医科大学心血管疾病基础研究重点实验室;宁夏医科大学科技中心;
【基金】：国家自然科学基金资助项目(81260063、81260105、81160044)
【分类号】：R329

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