大鼠椎骨错缝模型不同时间节点全血黏度变化的实验研究

发布时间：2018-02-26 16:45

本文关键词： 腰椎椎骨血液黏度血瘀　出处：《中国骨伤》2014年12期 　论文类型：期刊论文

【摘要】：目的 :通过检测大鼠腰椎椎骨错缝模型不同时间点全血黏度的变化,研究石氏伤科气血理论及骨错缝、筋出槽学说,揭示慢性脊柱病损的病理生理学特征。方法:将36只350~450 g的SPF级雄性SD大鼠随机分为旋转固定组(RF组)、单纯固定组(SF组)和假手术组(Sham组),每组12只。RF组和SF组大鼠腰椎L4-L6节段植入椎体外部连接固定装置,RF组大鼠使L5棘突向右侧旋转,造成L5棘突与L4和L6棘突的不共线;SF组大鼠单纯植入椎体外部连接固定装置不进行旋转。分别于固定后1、4、8和12周时,检测各组大鼠的全血黏度变化。结果:固定4、8周之后,RF组和SF组大鼠在高切(150/s)、中切(60/s)和低切变速率(10/s)下的全血黏度皆高于Sham组大鼠(P0.05)。固定1、12周后各组大鼠全血黏度比较差异无统计学意义(P0.05)。结论 :椎体骨错缝、筋出槽可导致大鼠全血黏度升高,增加血脉瘀阻的程度,从而进一步诱发或加重脊柱病损的发生。
[Abstract]:Objective: to study the whole blood viscosity of rat lumbar vertebrae fracture model at different time points in order to study the theory of Qi and blood and bone suture. To reveal the pathophysiological characteristics of chronic spinal lesions. Methods: 36 male Spinal Spinal Spinal lesion (SPF) rats were randomly divided into rotating fixation group (RF group) and simple fixation group (SF group) and sham-operated group (12 rats in each group. RF group and SF group). L4-L6 segment of lumbar vertebrae was implanted with external spinal junction fixator. In RF group, L5 spinous process rotated to the right. L5 spinous process and L4 and L6 spinous process were not rotated in SF group. Results: after 4 weeks of fixation, the whole blood viscosity of RF group and SF group were higher than that of Sham group at high shear rate of 150 / s (60 / s) and low shear rate (10 / s). After 12 weeks of fixation, the whole blood viscosity of rats in the RF group and SF group was higher than that in the Sham group (P 0.05). After 12 weeks of fixation, the whole blood viscosity of the rats in the RF group and the SF group was significantly higher than that in the control group. There was no significant difference in degree between the two groups (P 0.05). It can cause the increase of whole blood viscosity, increase the degree of blood vessel stasis, and further induce or aggravate the occurrence of spinal disease.
【作者单位】：上海中医药大学附属曙光医院石氏伤科医学中心上海市中医药研究院骨伤科研究所;
【基金】：国家自然基金青年基金项目(编号:81202707) 高等学校博士学科点专项科研基金(编号:新教师类)(编号:20123107120006) 上海高校青年教师培养资助计划(编号:ZZszy13024) “海派中医流派传承研究基地”(编号:ZYSNXD-CC-HPGC-JD-001) 上海高校创新团队建设项目(编号:2009-26)~~
【分类号】：R-332;R681.5

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