腺病毒介导碱性成纤维细胞生长因子对人脐静脉内皮细胞生物学行为的影响及血管瘤动物模型建立的初步研究

发布时间：2018-02-27 16:22

本文关键词： 血管瘤血管生成碱性成纤维细胞生长因子血管内皮细胞生长因子腺病毒动物模型　出处：《第二军医大学》2007年博士论文　论文类型：学位论文

【摘要】： 目的: bFGF基因以腺病毒为载体体外转染HUVEC,改变其生物学行为,并在裸鼠体内发挥促血管生成作用,旨在构建血管瘤动物模型。方法:应用同源重组的方法构建携带bFGF基因的腺病毒载体,体外转染HUVEC。检测bFGF和VEGF蛋白、mRNA表达变化。观察bFGF基因转染的HUVEC细胞增殖能力的变化,及在裸鼠体内促血管生成作用。结果:构建了携带bFGF基因的腺病毒载体,并成功在HUVEC表达,同时上调VEGF表达。过量表达bFGF和VEGF的HUVEC细胞增殖能力增强,并在裸鼠体内促进血管增生。结论:通过基因工程的方法可以获得高表达bFGF的HUVEC细胞,该基因可引起细胞生物学行为发生改变,在裸鼠体内可以诱导血管瘤样病变形成,为建立真正的血管瘤动物模型奠定基础。
[Abstract]:Objective: to transfect bFGF gene into HUVECs by adenovirus in vitro, to change its biological behavior and to promote angiogenesis in nude mice. Methods: adenovirus vector carrying bFGF gene was constructed by homologous recombination method and transfected into HUVECs in vitro. The expression of bFGF and VEGF protein was detected. The proliferative ability of HUVEC cells transfected with bFGF gene was observed. Results: the adenovirus vector carrying bFGF gene was successfully expressed in HUVEC and up-regulated VEGF expression. The proliferative ability of HUVEC cells with overexpression of bFGF and VEGF was enhanced. Conclusion: HUVEC cells with high expression of bFGF can be obtained by genetic engineering, which can induce the change of cell biological behavior and induce angiomatous lesions in nude mice. To establish a true animal model of hemangioma.
【学位授予单位】：第二军医大学
【学位级别】：博士
【学位授予年份】：2007
【分类号】：R543;R-332;R732.2

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