分子生物学技术在肠道菌群研究中的进展

发布时间：2018-02-27 23:18

本文关键词： 肠道菌群荧光原位杂交基于聚合酶链式反应的变性梯度凝胶电泳实时荧光定量PCR 基因芯片焦磷酸测序　出处：《微生物学通报》2014年06期 　论文类型：期刊论文

【摘要】：人的肠道中栖息着大量微生物,这些微生物与宿主形成一个相互依赖且相互制约的微生态系统。肠道菌群结构与遗传、饮食、疾病、环境等因素存在千丝万缕的联系,其地位与作用相当于一个后天获得的"器官",对人体的消化、营养吸收、能量供应、脂肪代谢、免疫调节、抗病等诸多方面有不可替代的作用,因此,研究人的肠道菌群具有十分重要的意义和作用。本综述着重论述荧光原位杂交(FISH)、基于聚合酶链式反应的变性梯度凝胶电泳(Polymerase chain reaction denaturing gradient gel electrophoresis,PCR-DGGE)技术、实时荧光定量PCR(Real-time polymerase chain reaction)技术、基因芯片(Gene chip)技术及以焦磷酸测序平台为代表的第二代测序技术等多种分子生物学技术在肠道菌群研究方面的应用,并对未来肠道菌群方面的研究进行展望。
[Abstract]:The human gut habitat in a large number of microorganisms, these microbes and the host to form a mutual dependence and mutual restriction of the micro ecosystem. The structure of intestinal flora and heredity, diet, disease, closely related to environmental factors, the position and function of the equivalent of a day after the "organ", on the human body the digestion and absorption of nutrients, energy supply, fat metabolism, immune regulation, has an irreplaceable role in many aspects such as disease resistance therefore, intestinal flora study plays an important role. This review focuses on the fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis and polymerase chain reaction (Polymerase based on chain reaction denaturing gradient gel electrophoresis PCR-DGGE) technology, real-time fluorescence quantitative PCR (Real-time polymerase chain reaction) technology, gene chip technology (Gene chip) and by pyrosequencing The platform is the application of second generation of sequencing technology and other molecular biological technology in the research of intestinal microflora, and prospects for future research on intestinal flora.

【作者单位】：内蒙古农业大学乳品生物技术与工程教育部重点实验室;
【基金】：国家863计划项目(No.2011AA100902) 内蒙古自治区应用技术研发资金计划项目(No.20110734)
【分类号】：R37

【参考文献】