尿液分离大肠埃希菌Ⅰ类整合子分子特征与耐药性的关系研究

发布时间：2018-03-03 21:44

  本文选题：Ⅰ类整合子　切入点：基因盒　出处：《中华医院感染学杂志》2014年01期 　论文类型：期刊论文

【摘要】：目的了解社区感染尿液分离大肠埃希菌中Ⅰ类整合子的分布,并分析其与细菌耐药性的关系。方法选取114株临床非重复分离自医院门诊患者尿液标本中的大肠埃希菌,经全自动细菌分析系统鉴定并检测其对临床常用抗菌药物的耐药性,聚合酶链反应(PCR)检测Ⅰ类整合酶基因,对Ⅰ类整合酶阳性菌株用PCR扩增并测序分析可变区基因盒种类。结果 114株临床非重复分离自尿液大肠埃希菌中有50株检测到Ⅰ类整合酶基因;共检测出两种不同长度的可变区片段:1 700bp片段(30株)为dfrA17-aadA5,2 100bp片段(5株)为dfrA12-orfF-aadA2,有两株大肠埃希菌同时扩增出1 700bp和2 100bp的可变区片段,有17株Ⅰ类整合酶基因阳性大肠埃希菌未扩增出可变区;114株大肠埃希菌对氨苄西林、磺胺甲VA唑/甲氧苄啶和左氧氟沙星的耐药率较高,50.0%,对头孢替坦、亚胺培南、厄他培南、哌拉西林/他唑巴坦、呋喃妥因、阿米卡星耐药率较低,5.0%;Ⅰ类整合子阳性菌株对氨苄西林、庆大霉素、妥布霉素和磺胺甲VA唑/甲氧苄啶的耐药率高于Ⅰ类整合子阴性菌株。结论与医院感染相比,社区感染尿液中分离的大肠埃希菌受抗菌药物的选择压力相对较小,其对常用抗菌药物的耐药率、Ⅰ类整合子携带率均较低,同时Ⅰ类整合子中携带的基因盒种类也较单一。
[Abstract]:Objective to investigate the distribution of class I integron in isolated Escherichia coli from community infected urine, and to analyze its relationship with bacterial resistance. Methods 114 strains of Escherichia coli were isolated from the urine samples of outpatients in hospital. The drug resistance to antimicrobial agents was identified by automatic bacterial analysis system, and the class I integrase gene was detected by polymerase chain reaction (PCR). Class I integrase positive strains were amplified by PCR and sequenced to analyze the type of variable region gene cassette. Results 50 of 114 clinical isolates of Escherichia coli were found to have class I integrase genes. Two kinds of variable region fragments of different length (30 strains of variable region: 1 700 BP) were identified as dfrA17-aadA5 (2100bp) and 5 strains of DfrA12-orfF-aadA2 were detected. Two strains of Escherichia coli amplified 1700bp and 2100bp variable region fragments at the same time. There were 17 strains of class I integrase gene positive Escherichia coli. 114 strains of Escherichia coli were resistant to ampicillin, sulfamethazol / trimethoprim and levofloxacin, and were resistant to ceftitan, imipenem and ertapenem. The drug resistance rate of piperacillin / tazobactam, furantoin, amikacin was lower than that of ampicillin, gentamycin. The resistance rate of tobramycin and sulfamethazol / trimethoprim was higher than that of class 鈪，

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