人生长激素真核表达载体的构建及其瞬时表达

发布时间：2018-03-06 15:18

本文选题：人生长激素　切入点：真核表达载体　出处：《郑州大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的人生长激素(human growth hormone，hGH)是由人脑垂体前叶分泌的一种多肽，可促进生长发育与调节物质代谢及免疫功能，对机体的各个器官和各种组织均有影响，尤其对骨骼、肌肉及内脏器官的作用更为显著。hGH基因位于第17号染色体长臂，包括5个外显子和4个内含子以及3′末端、5′末端的不翻译序列，跨度为26kb。人生长激素是由hGH基因先翻译为激素原，然后经酶解将217个氨基酸的前生长素切去26个氨基酸的前肽转变而成[3，4]。人生长激素是一条单链，含有191个氨基酸的亲水性球蛋白，分子量为21.7KDa。最近研究发现，生长激素缺乏症远比我们过去认为的更加普遍。成人生长激素缺乏症是一种非常独特的疾病，它对心血管危险因子有一定的影响(如引起高脂血症、降低纤维蛋白溶解作用、发生动脉粥样硬化的概率增加)，使心脏的结构和功能发生异常改变，增加了心血管疾病的罹患率，并可使机体发生畸形构建(降低机体肌肉量、增加脂肪含量、使细胞外水分减少)，身体活动能力下降，，损害骨骼的强度，区域性骨矿物质密度下降；儿童生长激素缺乏还可以导致侏儒症。总之，生长激素缺乏是一种危害人类健康的不可小觑的疾病。研究证明牛生长激素对人类无效，而从尸体中获得的人生长激素的量少且用于临床治疗时可使Creutzfeldt-Jacob病的患病率增高，自从运用重组DNA技术将人生长激素基因在大肠埃希菌中表达以来，重组人生长激素在临床中就有着广泛的应用。对生长激素缺乏症的儿童来说，用重组人生长激素进行替代治疗效果显著，其可以使患者成年终身FAH高最接近遗传靶身高；在治疗成
[Abstract]:Purpose. Human growth hormone growth hormonehGH is a polypeptide secreted by the anterior pituitary of human brain. It can promote growth and development and regulate the metabolism of substances and immune function. It has an effect on all organs and tissues of the body, especially on bone. The effects of muscle and visceral organs were more significant in the long arm of chromosome 17, including 5 exons, 4 introns and 3 'terminal untranslated sequences. The span of human growth hormone is 26kb.Human growth hormone is translated into prohormone by hGH gene, and then digested with 217 amino acid proauxin to transform the prepeptide of 26 amino acids into [3n4]. Human growth hormone is a single strand. A hydrophilic globulin containing 191 amino acids with a molecular weight of 21.7 KDa. Recent studies have found that growth hormone deficiency is far more common than we previously thought. Adult growth hormone deficiency is a very unique disease that has an impact on cardiovascular risk factors (such as hyperlipidemia). Reduce fibrinolysis, increase the probability of atherosclerosis, cause abnormal changes in the structure and function of the heart, increase the incidence of cardiovascular disease, and cause malformation of the body. Increase fat content, reduce extracellular water, reduce physical activity, damage bone strength, decrease regional bone mineral density, and childhood growth hormone deficiency can also lead to dwarfism. Growth hormone deficiency is a serious disease that endangers human health. Studies have shown that bovine growth hormone does not work in humans, and that human growth hormone obtained from cadavers can increase the prevalence of Creutzfeldt-Jacob 's disease when it is used for clinical treatment. Since the recombinant DNA technique was used to express the human growth hormone gene in Escherichia coli, recombinant human growth hormone has been widely used in clinical practice. The effect of recombinant human growth hormone replacement therapy is significant, which can make adult lifetime FAH height closest to genetic target height.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R346

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