大肠杆菌表达的病毒样颗粒疫苗

发布时间：2018-03-06 19:37

本文选题：疫苗　切入点：病毒样颗粒　出处：《生命的化学》2014年01期 　论文类型：期刊论文

【摘要】：重组病毒样颗粒是病毒衣壳蛋白外源表达的重要形式,形态结构与天然病毒高度相似,位于纳米尺度的大小易于被免疫系统识别,可激发机体产生保护性免疫反应,且不含有病毒基因,因此,是一种理想的疫苗形式,也是基于结构进行疫苗设计的重要结构载体。目前已上市的乙型肝炎疫苗、人乳头瘤病毒疫苗和戊型肝炎疫苗等基因工程疫苗均采用病毒样颗粒形式。大肠杆菌表达系统被广泛用于基因工程药物的生产,具有安全性好、生产周期短、易于放大生产等优点,在病毒样颗粒疫苗应用上具有良好前景。本文综述了利用大肠杆菌研制戊型肝炎疫苗和人乳头瘤病毒疫苗的进展,特别是这些病毒样颗粒疫苗的表达及组装、表位结构特征和临床试验结果。
[Abstract]:Recombinant virus-like particles are an important form of exogenous expression of virus capsid protein. The morphology and structure of recombinant virus particles are similar to those of natural viruses. The size of recombinant virus particles at nanometer scale is easy to be recognized by the immune system, which can stimulate the body to produce protective immune response. And does not contain the virus gene, therefore, is a kind of ideal vaccine form, also is the important structural vector which carries on the vaccine design based on the structure. The recombinant vaccines such as human papillomavirus vaccine and hepatitis E vaccine are in the form of virus-like particles. Escherichia coli expression system is widely used in the production of genetically engineered drugs with good safety and short production cycle. Because of its advantages such as easy to enlarge production, it has a good prospect in the application of virus-like granular vaccine. This article reviews the development of hepatitis E vaccine and human papillomavirus vaccine using Escherichia coli. In particular, the expression and assembly, epitope structure and clinical trial results of these virus-like granular vaccines.
【作者单位】：厦门大学国家传染病诊断试剂与疫苗工程技术研究中心;
【基金】：国家自然科学基金项目(30925030;81172885)
【分类号】：R392

【共引文献】