建立兔腰椎间盘退变模型的实验研究

发布时间：2018-03-07 10:13

本文选题：椎间盘退变　切入点：动物模型　出处：《福建医科大学》2007年硕士论文　论文类型：学位论文

【摘要】： 【目的】:探讨纤维环刺伤法诱导兔腰椎间盘退变建立动物模型的可行性,建立一种缓慢进展、可重复性好的兔腰椎间盘退变的动物模型,适用于研究椎间盘退变的病因学、病理生理学,适用于检验可靠、有效的治疗椎间盘退变的新方法,例如生长因子疗法、基因疗法、细胞疗法、组织工程等。【方法】:24只普通级雄性新西兰大白兔(重量约3.0kg,1岁龄),手术取右侧腹膜后入路,用16号穿刺针于L2/3椎间盘纤维环右前外侧刺入,深度为5mm,分别于术前及术后4、8、16周对造模的椎间盘(L2/3)及正常椎间盘(L4/5)进行磁共振(MRI)检查,动物处死取材后行组织学及免疫组化观察。【结果】:①术前到术后16周组,造模组椎间盘毮核MRI T2W1信号呈逐渐减弱趋势(P0.01);②组织学观察到毮核脊索细胞逐渐减少,并逐渐被纤维软骨组织替代,纤维环与毮核之间失去明显的界限;③免疫组化观察到毮核中II型胶原表达较对照组逐渐减少(P0.01),而I型胶原表达较对照组逐渐增多(P0.01)。【结论】:1.本实验通过MRI影像学分析、组织学观察及免疫组化观察证实了兔椎间盘退变的基本特征;2.纤维环刺伤法可以诱导兔椎间盘缓慢退变,为椎间盘退变的进一步研究提供动物模型。
[Abstract]:[objective]: to investigate the feasibility of rabbit lumbar disc degeneration induced by fibrous ring puncture, and to establish a slowly progressive and reproducible animal model of lumbar disc degeneration, which is suitable for the study of the etiology of intervertebral disc degeneration. Pathophysiology is suitable for testing reliable and effective new methods for the treatment of intervertebral disc degeneration, such as growth factor therapy, gene therapy, cell therapy, tissue engineering and so on. [methods] Twenty-four normal male New Zealand white rabbits (weighing about 3.0 kg / 1 years old) were operated on the right retroperitoneal approach and punctured on the right anterolateral side of the L 2 / 3 intervertebral disc fiber ring with 16 puncture needle. The depth was 5 mm. MRI was performed on L2 / 3 of model intervertebral disc and L4 / 5 of normal intervertebral disc before operation and 8 weeks after operation. The animals were sacrificed for histological and immunohistochemical observation. [results] the signal intensity of MRI T2W1 in the intervertebral disc nucleus of the model group decreased gradually from pre-operation to 16th week after operation. Histologically, the notochordal cells of the nucleus carinii were gradually decreased and replaced by fibrochondrocytes. The expression of type II collagen was decreased gradually compared with the control group, while the expression of type I collagen was gradually increased than that of the control group. [conclusion] 1. The basic features of disc degeneration in rabbits were confirmed by MRI imaging analysis, histological observation and immunohistochemical observation. 2. The method of fibrous ring puncture can induce slow degeneration of intervertebral disc in rabbits. To provide animal models for further study of disc degeneration.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R681.55;R-332

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