犬经皮插管肾动脉注射人HGF基因的实验研究

发布时间：2018-03-08 10:38

本文选题：介入插管技术　切入点：肾动脉　出处：《南京医科大学学报(自然科学版)》2005年08期 　论文类型：期刊论文

【摘要】：目的:运用介入插管技术,经犬肾动脉快速注射含人肝细胞生长因子(HGF)重组基因的质粒(pCMV鄄HGF)。检测不同时间犬肾脏组织中外源性人HGF蛋白的水平,了解外源性HGF基因在犬肾脏组织内的表达情况。方法:健康成年犬6只,在麻醉和无菌条件下均经左肾动脉介入插管。其中4只注射pCMV鄄HGF质粒,1只注射空质粒(pcDNA3)对照,1只为注射等渗盐水空白对照。注射后不同时间留取犬静脉血以及部分肾组织,采用ELISA方法,分别检测血清及肾组织中人HGF蛋白的水平。结果:①该实验建立的检测人重组HGF的ELISA敏感性高(15～1000ng/ml),重复性强;②接受3种不同处理的犬静脉血中均未检测到人重组HGF蛋白的存在;③注射pCMV鄄HGF质粒的犬肾组织中能够检测到人重组HGF蛋白的存在。72h肾组织中HGF的水平范围在27～667ng/mg蛋白之间。注射空质粒和等渗盐水的犬(即2号和5号犬)肾组织则无表达。结论:运用介入插管技术,经犬肾动脉快速注射pCMV鄄HGF可以在肾脏内产生基因高转染表达。
[Abstract]:Objective: using interventional catheterization technique, the recombinant plasmid pCMV-HGF containing human hepatocyte growth factor (HGF) gene was injected into the canine renal artery rapidly. The levels of exogenous human HGF protein in the canine kidney tissue were detected at different times. Objective: to investigate the expression of exogenous HGF gene in canine kidney. Under anaesthesia and aseptic conditions, interventional catheterization of left renal artery was performed. Among them, 4 were injected with pCMV-HGF plasmid, 1 was injected with empty plasmid pcDNA3) and 1 was injected with isoosmotic saline. The venous blood and some renal tissues were collected at different time after injection. Using ELISA method, Results the ELISA sensitivity of human recombinant HGF was 151000 ng / ml, and the reproducibility of human recombinant HGF eggs was not detected in the venous blood of dogs treated with three different treatments. Human recombinant HGF protein was detected in the kidney tissue of dogs injected with pCMV-HGF plasmid. 72 h after injection, the level of HGF in renal tissue was in the range of 27667 ng / mg protein. Dogs injected with empty plasmids and isosmotic saline (I. e., 2 and 5 dogs) showed that the level of HGF in renal tissue was in the range of 27667 ng / mg. There was no expression in renal tissue. Conclusion: interventional intubation, Rapid injection of pCMV-HGF into the renal artery can produce high-transfection expression in the kidney.
【作者单位】：南京医科大学第一附属医院介入放射科南京医科大学第一附属医院介入放射科南京医科大学第一附属医院肾内科
【分类号】：R692.5

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