人胎儿骨髓间充质干细胞的生物学特性研究

发布时间：2018-03-09 06:14

本文选题：间充质干细胞　切入点：造血干细胞　出处：《苏州大学》2006年硕士论文　论文类型：学位论文

【摘要】： 间充质干细胞(MSC)是一种具有多向分化功能的细胞群体,早期主要从成人骨髓中分离获得,近年研究表明,多种组织中均含有MSC如外周血、脐带血、脂肪组织、羊水以及其他的一些胎儿组织。MSC在体外能够扩增,其在适合的条件下,可向包括脂肪细胞、成骨细胞、软骨细胞、心肌细胞、神经细胞、肝细胞的多种组织细胞分化。MSC具有免疫调节作用,MSC诱导淋巴细胞通过分泌可溶性细胞因子而产生免疫抑制效应,在混合淋巴细胞培养中能抑制T细胞的增殖,与造血干细胞共输可降低移植物抗宿主病的发生率。最近人们发现它能够逆转内脏和肝脏的4期急性GVHD。鉴此MSCs作为造血重建的促进剂,预防和治疗GVHD,它们在器官移植排斥的治疗和自身免疫性疾病的组织修复中具有潜在的应用价值。本实验应用细胞生物学和免疫学等多种技术: (1)分离培养并鉴定人胎儿源骨髓间充质干样细胞(FMSCs) ;(2)观察不同剂量的射线辐照对增殖;细胞周期的影响,比较其在放射损伤前后的生物学特性,辐照后成脂、成骨分化的潜能;(3)研究人胎儿源骨髓间充质干细胞在免疫调节中的作用。目的:分离培养并鉴定人胎儿源骨髓间充质样干细胞(FMSCs) ;比
[Abstract]:MSC (Mesenchymal Stem Cell) is a multifunctional cell population, which was isolated from adult bone marrow in the early stage. Recent studies have shown that many tissues contain MSC, such as peripheral blood, umbilical cord blood, adipose tissue. Amniotic fluid and some other fetal tissues, such as. MSC, can be expanded in vitro to include adipocytes, osteoblasts, chondrocytes, cardiomyocytes, nerve cells, and, where appropriate, adipocytes, osteoblasts, chondrocytes, cardiomyocytes, nerve cells. MSC can induce lymphocytes to produce immunosuppressive effect by secreting soluble cytokines, which can inhibit the proliferation of T cells in mixed lymphocyte culture. Coadministration with hematopoietic stem cells can reduce the incidence of graft-versus-host disease. It has recently been found to be able to reverse phase 4 acute GVHDs of viscera and liver. This MSCs is used as an accelerator for hematopoietic reconstitution. The prevention and treatment of GVHD, which have potential application value in the treatment of organ transplantation rejection and tissue repair of autoimmune diseases. In this study, cell biology and immunology were used to observe the effects of different doses of radiation irradiation on proliferation and cell cycle of human fetal bone marrow mesenchymal mesenchymal stem cells (FMSCs2). To study the role of human fetal bone marrow mesenchymal stem cells in immunomodulation by comparing their biological characteristics before and after radiation injury and the potential of adipogenesis and osteogenic differentiation after irradiation. Objective: to isolate and identify human fetal bone marrow mesenchymal stem cells (MSCs).
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

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