硒代蛋氨酸对小鼠巨噬细胞增殖及硒蛋白K的影响

发布时间：2018-03-09 13:04

本文选题：硒代蛋氨酸　切入点：巨噬细胞　出处：《中国热带医学》2014年11期 　论文类型：期刊论文

【摘要】：目的探究硒代蛋氨酸(Selenomethionine,Se-Met)对小鼠巨噬细胞的增殖及巨噬细胞中硒蛋白K(Selenopro-tein K,Sel K)的影响,并观察Sel K在小鼠巨噬细胞中的定位。方法加硒代蛋氨酸(Se-Met)培养巨噬细胞,用CCK-8法检测巨噬细胞的增殖活力,采用逆转录-聚合酶链反应(RT-PCR)和蛋白质免疫印迹技术检测Se-Met对小鼠巨噬细胞中Sel K m RNA和Sel K蛋白表达的影响;用细胞免疫荧光法观察Sel K在巨噬细胞中的位置。结果 Se-Met能促进巨噬细胞的增殖,其中浓度为1μmol/L Se-Met在处理巨噬细胞24 h和48h后,能显著性地促进巨噬细胞的增殖(P0.01);1μmol/L Se-Met能使巨噬细胞中Sel K的m RNA含量显著增加(24 h处理组,P0.05;48 h处理组,P0.01),另外,Sel K蛋白的含量也有所增加(48 h处理组,P0.05)。浓度为100μmol/L Se-Met在处理巨噬细胞48 h后,能极显著性地抑制巨噬细胞增殖(P0.01)。结果还显示Sel K蛋白与内质网标志物KDEL都存在于巨噬细胞内的相同位置上。结论 Se-Met在促进巨噬细胞增殖的同时,使存在于内质网上的Sel K增加。
[Abstract]:Objective to investigate the effect of selenomethionine selenomethionine Se-Met( Se-Met) on the proliferation of mouse macrophages and the effect of selenopro-tein Kel K in mouse macrophages, and to observe the localization of Sel K in mouse macrophages. The proliferative activity of macrophages was detected by CCK-8 assay. The effects of Se-Met on the expression of Sel K m RNA and Sel K protein in mouse macrophages were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The position of Sel K in macrophages was observed by immunofluorescence assay. Results Se-Met could promote the proliferation of macrophages, including 1 渭 mol/L Se-Met after 24 h and 48 h treatment of macrophages. It can significantly promote the proliferation of macrophages P0.01G 1 渭 mol/L Se-Met can significantly increase the content of m RNA of Sel K in macrophages of 24 h treatment group, P0.0548 h treatment group P0.01N, and the protein content of Sel K protein also increase 48 h treatment group P0.055.The concentration of Sel K protein is 100 渭 mol/L Se-Met. After 48 h treatment of macrophages, The results also showed that Sel K protein and endoplasmic reticulum marker KDEL existed in the same position in macrophages. Conclusion Se-Met can promote the proliferation of macrophages at the same time. Increase the number of Sel K in endoplasmic reticulum.
【作者单位】：汕头大学医学院;深圳市疾病预防控制中心;
【基金】：国家自然科学基金(No.81273075)
【分类号】：R329.2

【参考文献】