不同剂量碘酸钾对大鼠生育力及其仔鼠大脑、小脑发育影响的形态学实验观察

发布时间：2018-03-13 18:26

本文选题：碘酸钾　切入点：大脑　出处：《天津医科大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的: 研究摄入不同浓度的碘酸钾对Wistar大鼠生育能力的影响及1日、20日和60日龄仔鼠大脑皮质、海马和小脑的形态学改变,进一步探讨碘缺乏和碘过量对大鼠生育能力和仔鼠大脑、小脑发育及功能的影响。方法: 将断乳后一个月,体重在120-140g的健康Wistar大鼠雌雄各半,随机分为6组:低碘组(LI)、适碘组(NI)、5倍碘组(5HI)、10倍碘组(10HI)、50倍碘组(50HI)、100倍碘组(100HI),饮用不同浓度的KIO_3碘水,饲养3个月后雌雄合笼,观察雌鼠受孕、生育及新生仔鼠的生长发育情况。取1日、20日和60日龄仔鼠大脑、小脑电子天平称重,中性福尔马林溶液固定后常规包埋,进行HE、镀银、焦油紫、免疫组化(NSE、GFAP、MBP)染色,观察大脑皮质内锥体细胞层锥体细胞和海马神经元、神经胶质细胞、少突胶质细胞及小脑Purkinje细胞、颗粒细胞的形态学改变。应用Motic Images Advanced 3.1图像分析系统测量以下指标:大脑皮质锥体细胞顶树突棘突密度、基树突数、初级树突分枝指数、MCSA、V_V、和N_A。海马NSE、GFAP、MBP阳性神经元及胶质细胞的N_A、平均灰度值及强阳性细胞率。仔鼠小脑皮质各层厚度、Purkinje细胞面积、树突覆盖面积、分枝密度、树突总长度、棘突密度及NSE、GFAP、MBP阳性神经元及胶质细胞的平均灰度值。结果: 1.大鼠生育力:5HI、10HI和50HI组各项生育指标与NI组相比无统计学意义;LI组雌鼠受孕率、产仔数明显低于NI组,孕鼠及仔鼠死亡率明显高于NI组;
[Abstract]:Objective:. To study the effects of different concentrations of potassium iodate on the fertility of Wistar rats and the morphological changes of cerebral cortex, hippocampus and cerebellum of rats born on 1st, 20th and 60 days, and to further explore the effects of iodine deficiency and iodine excess on the fertility of rats and their brains. Effects of cerebellum development and function. Methods:. One month after weaning, healthy Wistar rats weighing 120-140 g were randomly divided into six groups: low iodine group (LII) group, high iodine group (5 times iodine group), 10 times iodine group (10 times iodine group) and 50 times iodine group (100 times iodine group), drinking different concentrations of KIO_3 iodine water, and then feeding for 3 months. To observe the growth and development of pregnant, fertile and newborn mice, the cerebellum and cerebellum were weighed by electronic balance on 1st, 20th and 60 days, fixed with neutral formalin solution, and then were routinely embedded for HEH, silver plating, tar violet, and so on. The pyramidal cell layer pyramidal cells, hippocampal neurons, glial cells, oligodendrocytes and cerebellar Purkinje cells were observed by immunohistochemical staining. Morphological changes of granulosa cells. The following indexes were measured by Motic Images Advanced 3.1 image analysis system: the density of apical dendritic spinous process and the number of basal dendrites in the pyramidal cells of cerebral cortex. Primary dendritic branching index (PDI): MCSA-V, and Nap.NA-, mean gray value and strong positive cell rate of MBP positive neurons and glial cells in hippocampus, thickness of Purkinje cells, dendritic covering area, branching density, total dendritic length in cerebellar cortex of neonatal rats, MBP positive neurons and glial cells in hippocampal NSEGAPAPBP-positive neurons and glial cells, the thickness of Purkinje cells, dendritic covering area, branch density, total dendritic length, The density of spinous process and the mean gray value of MBP positive neurons and glial cells in NSEG GFAPN. Results:. 1. Compared with NI group, the fertility of 10 HI and 50 HI groups had no statistical significance. The pregnant rate and litter size of female mice in Li group were significantly lower than those in NI group, and the mortality of pregnant rats and their offspring was significantly higher than that of NI group.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

【相似文献】