生物碱对胰蛋白酶作用的光谱变化

发布时间：2018-03-14 05:46

本文选题：胰蛋白酶　切入点：葛根素　出处：《四川大学》2005年硕士论文　论文类型：学位论文

【摘要】：胰蛋白酶是一种动物来源的蛋白水解酶,具有高度的专一性,只水解L-型精氨酸和L-型赖氨酸的羧基组成的肽键。胰蛋白酶是所有胰脏蛋白酶原的共同激活剂(包括其自身的前体胰蛋白酶原)。作为一种重要的消化酶,胰蛋白酶在医药、食品、工业及有机合成中都有着广泛的应用,人们也对其进行了深入的研究。现在的热点主要集中于天然胰蛋白酶抑制剂。生物碱是一类含氮杂环的碱性天然有机物,广泛存在于植物体内,大多数具有重要的医疗价值。本文基于生物碱的药用价值,借助近年来发展迅速的光谱分析方法探讨了三种生物碱小分子与胰蛋白酶的相互作用。本文选择的三种生物碱分别为:葛根素、秋水仙碱和喜树碱。葛根素(puerarin)是由豆科植物野葛(pueraria lobata)甘蔼藤(puerarin thomsonii)根中提取的一种黄酮苷,具有广泛的药理作用,临床上主要用于心脑血管疾病的治疗;秋水仙碱(colchicine)是从百合科植物秋水仙(Colchicumautumnale L.)的球茎中分离到的一种卓酚酮类生物碱,可用于治疗痛风,对某些恶性肿瘤亦有较好的疗效;喜树碱(camptothecin,简称CPT)是从我国特有的天然植物喜树(camptotheca acuminate)果实中分离出的一种具
[Abstract]:Trypsin is an animal-derived proteolytic enzyme with high specificity. A peptide bond that hydrolyzes only the carboxyl groups of L-arginine and L-lysine. Trypsin is a common activator of all trypsinogen (including its own precursor, trypsinogen). As an important digestive enzyme, trypsin is used in medicine. Food, industry and organic synthesis have been widely used and have been studied deeply. Nowadays, the focus is mainly on natural trypsin inhibitors. Alkaloids are a class of natural alkaloids containing nitrogen heterocycles, which are widely found in plants, most of which have important medical value. The interaction of three small alkaloid molecules with trypsin has been studied by means of the rapidly developed spectral analysis method in recent years. The three alkaloids selected in this paper are puerarin, colchicine and camptothecin. Puerarin is a flavonoid glycoside extracted from the roots of Pueraria lobata), which has a wide range of pharmacological effects, such as puerarin thomsoniiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiii. Colchicine is an alkaloid isolated from the corm of Colchicum autumnale L., which can be used in the treatment of gout and has good curative effect on some malignant tumors. Camptothecin (CPT) was isolated from the fruit of camptotheca acuminate, a natural plant endemic to China.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R285;Q50

【引证文献】