小鼠子宫内膜中DBA、ECL和RCA-Ⅰ受体在胚泡植入过程中的表达状况的实验研究

发布时间：2018-03-14 05:24

本文选题：植入　切入点：凝集素受体　出处：《山东大学》2005年硕士论文　论文类型：学位论文

【摘要】：目的:胚胎着床是一个十分复杂的过程,是妊娠的关键所在,它涉及胚胎的发育分化、子宫内膜的同步变化、胚泡对于子宫内膜的黏附、侵入及子宫内膜的包埋等环节。近年来的研究表明,胚泡着床过程不仅受到神经内分泌因素的调节,而且还与胚泡和子宫内膜产生的多种调节因子有关,凝集素受体即是这些调节因子的一员。凝集素受体是指分布于细胞表面的糖蛋白或糖脂。凝集素受体上具有能被凝集素特异识别的糖基。它们在细胞的生长、分化、细胞之间的黏附及相互识别中起重要作用。为了探讨子宫内膜的凝集素受体与胚泡着床的关系,我们以早孕小鼠为研究对象,用亲和细胞化学染色的方法检测植入过程中子宫内膜上皮相关凝集素受体分布状况和变化规律,以明确与胚泡着床相关的凝集素受体并阐明它们在着床中的生物学效应,从而丰富生殖医学中有关着床的理论,并为新型抗着床避孕方法的研究提供重要的实验依据。研究方法:将健康育龄期雌性昆明小鼠行单侧输卵管结扎,2周后与正常有生育能力的雄鼠按2:1合笼交配,次日晨查见阴栓者定为孕第一天。分别于孕第4、6、7、8、9天颈椎脱臼处死孕鼠并立即剖腹取子宫(未孕侧作为对照侧)。所得标本用4%多聚甲醛固定,常规石蜡包埋,做厚度为6um的连续切片。采用SABC亲和细胞化学法检测DBA、ECL、RCA-Ⅰ三种凝集素的受体在着床期间的子宫内膜的分布情况。结果: 1 DBA染色结果:在怀孕一侧,DBA受体主要表达于胚胎滋养层细胞,子宫内
[Abstract]:Objective: embryo implantation is a very complex process, which is the key of pregnancy. It involves the development and differentiation of embryo, the synchronous change of endometrium, the adhesion of embryo bubble to endometrium. Recent studies have shown that the implantation process of blastocyst is not only regulated by neuroendocrine factors, but also related to many regulatory factors produced by blastocyst and endometrium. Lectin receptors are one of these regulatory factors. Lectin receptors are glycoproteins or lipids distributed on the surface of cells. Lectin receptors have glycosyl groups that can be specifically recognized by lectins. They grow and differentiate in cells. In order to study the relationship between endometrial lectin receptor and blastocyst implantation, we studied early pregnant mice. The distribution and changes of endometrial epithelium-associated lectin receptors during implantation were detected by affinity cytochemical staining in order to identify the lectin receptors associated with blastocyst implantation and to elucidate their biological effects in implantation. It can enrich the theory of implantation in reproductive medicine and provide an important experimental basis for the study of new anti-implantation contraceptive methods. Methods: female Kunming mice of healthy reproductive age were treated with unilateral tubal ligation for 2 weeks to mate with normal fertile male mice in 2: 1 cage. The first day of pregnancy was found in the next morning. The pregnant rats were killed by cervical dislocations on the 4th day of pregnancy, and the uterus was immediately removed by laparotomy. The specimens were fixed with 4% polyformaldehyde and were routinely embedded in paraffin wax. SABC affinity cytochemistry was used to detect the distribution of three lectin receptors in the endometrium during implantation. Results:. 1 DBA staining: the DBA receptor was mainly expressed in trophoblast cells and uterus on the pregnant side.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R321

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