豫医无毛小鼠近交系的繁殖特性及保种的研究

发布时间：2018-03-15 12:22

本文选题：豫医无毛小鼠　切入点：繁殖　出处：《郑州大学》2006年硕士论文　论文类型：学位论文

【摘要】：豫医无毛小鼠(Yuyi hairless mice，YYHL)是郑州大学实验动物中心发现并培育的我国第一个无毛小鼠近交系，主要表现为出生时被毛生长正常，14～21日龄开始从头部向下脱毛，1周左右除胡须外全部脱光，并终身保持无毛状态。在培育过程中发现该小鼠皮肤过早老化，行动迟缓，胸腺过早萎缩，寿命明显缩短(仅为10个月左右)以及免疫和生育异常等，同时无毛雌鼠受孕率较低，母性差，不能很好地护理它们的幼仔，幼鼠很难成活。为保护这一突变系小鼠，从21代开始对豫医无毛小鼠分离近交系的繁殖学特性、生殖器官进行研究；同时对雌性无毛小鼠实行超数排卵，，找出超排卵的最佳激素剂量；并对获得的胚胎进行冷冻、复苏、移植，比较不同条件下无毛小鼠的胚胎冷冻、复苏、移植的效果，找出最佳方法，为该分离近交系保种、育种、胚胎库的构建以及在生物医学领域的应用提供理论依据。方法： 1、对豫医无毛小鼠21代到29代，分别采用不同的交配方式，统计分析生育力、后代的胎数、产仔数、离乳率等繁殖指标。 2、对同龄的豫医无毛小鼠、有毛小鼠生殖器官进行解剖形态比较；同时对它们的卵巢、睾丸及乳腺的组织学比较，用10％的福尔马林固定，组织学切片，
[Abstract]:Yuyi hairless miceo YYHL) is the first hairless mouse inbred strain discovered and cultivated in Zhengzhou University Experimental Animal Center. The main manifestation was that the hair grew normally at birth at 14 days and 21 days of age, all except the beard were stripped off for about 1 week from the head down, and remained hairless for life. It was found that the skin of the mouse aged prematurely and moved slowly during the incubation process. Thymus premature atrophy, significantly shortened life span (only about 10 months), and abnormal immune and fertility, etc. At the same time, hairless female mice have low pregnancy rate and poor motherhood, so they can not take good care of their young. It is very difficult for young mice to survive. In order to protect the mutant mice, the reproductive characteristics and reproductive organs of the isolated inbred lines of Yuyi hairless mice were studied from the 21st generation, and the superovulation of female hairless mice was carried out. Find out the best hormone dose for superovulation, and freeze, resuscitate, transfer the embryos, compare the effects of freezing, resuscitation, and transplantation in hairless mice under different conditions, and find out the best way to preserve and breed the isolated inbred lines. The construction of embryo bank and its application in biomedical field provide theoretical basis. Methods:. 1. From 21 to 29 generations of Yuyi hairless mice, different mating methods were used to analyze the reproductive indexes such as fertility, the number of offspring, the number of offspring, the rate of milk separation and so on. 2. The anatomical morphology of hairy organs of Yuyi hairless mice of the same age and the histological comparison of their ovaries, testis and mammary glands were compared with 10% formalin.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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