“椎骨错缝”模型大鼠后肢H反射变化研究

发布时间：2018-03-18 00:11

本文选题：椎骨错缝　切入点：刚度　出处：《上海中医药大学学报》2014年06期 　论文类型：期刊论文

【摘要】：目的:观察腰椎"椎骨错缝"模型大鼠造模节段生物力学及H反射潜伏期的变化。方法:采用椎体外部连接固定系统(ELFS)诱导"椎骨错缝"大鼠模型。将160只350~450 g雄性SD大鼠随机分为正常对照组、假手术组、模型对照组和实验组。实验组通过外科手术将ELFS的棘突附着部件(SAU)与大鼠L4、L5和L6棘突相连接,再使用ELFS的外部连接部件将SAU连接起来;模型对照组仅植入SAU,不安装外部连接部件;假手术组仅进行手术干预,不植入任何部件;正常对照组不进行任何干预。再将各组按固定时间分为1、2、4、8和12周5个亚组。在各连接期结束时,脊柱刚度测试系统检测实验组和模型对照组固定节段刚度;肌电诱发电位仪检测各组大鼠后肢胫神经H反射。结果:"椎骨错缝"模型大鼠脊柱造模节段刚度随连接时间的延长而增加;且造模8周后,"椎骨错缝"模型大鼠后肢胫神经H反射潜伏期显著增加。结论:"椎骨错缝"模型大鼠相应造模节段发生了相关的生物力学及神经传导特性的改变。
[Abstract]:Objective: to observe the changes of segmental biomechanics and H reflex latency of lumbar vertebrae dislocation suture model in rats. Methods: the model of "vertebral suture" was induced by the external fixation system of vertebral body, ELFS. 160 rats of 350 pieces 450 g were used. Male SD rats were randomly divided into normal control group, Sham-operated group, model control group and experimental group. The experimental group connected the spinous process attachment part of ELFS with L4, L5 and L6 spinous process by surgical operation, and then connected SAU with the external connective part of ELFS. The model control group was only implanted with sau and no external connectors were installed, while the sham-operation group only performed surgical intervention and did not implant any parts. Each group was divided into 5 subgroups according to the fixed time. At the end of each connecting period, the stiffness of the experimental group and the model control group were measured by the spinal stiffness test system. The tibial nerve H reflex of the hind limbs of rats in each group was detected by electromyoelectric potentiometer. Results: the stiffness of spinal segment of the model rats increased with the extension of the connecting time. After 8 weeks of modeling, the latency of H reflex of tibial nerve in the posterior limb of the model rats increased significantly. Conclusion: the biomechanical and nerve conduction characteristics of the corresponding segment of the model have been changed in the rat model of "vertebral dislocation suture".
【作者单位】：上海中医药大学附属岳阳中西医结合医院;上海市中医药研究院推拿研究所;
【基金】：国家自然科学基金面上项目(81273869,81072891) 上海市高校青年教师培养计划资助项目(ZZszy12050) 国家中医药管理局重点学科项目国家临床重点专科建设项目上海市中医药事业发展三年行动计划项目(ZYSNXD-CC-HPGC-JD-011)
【分类号】：R274;R-332

【参考文献】