胎儿肠壁结缔组织对骨髓间充质干细胞分化的诱导

发布时间：2018-03-19 03:25

本文选题：骨髓间充质干细胞　切入点：DAPI　出处：《重庆医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 目的骨髓间充质干细胞(Bone marrow mesenchymal stem cells, Bm-MSCs)具有高度增殖、自我更新能力,可向三个胚层的细胞分化[1],在机体组织损伤修复过程及细胞移植治疗等多个领域有广阔的应用前景。目前,已有成功诱导Bm-MSCs分化为成骨细胞、软骨细胞、脂肪细胞、神经细胞、肝细胞、皮肤上皮细胞等的报道[2~7],尚未见肠壁结缔组织影响Bm-MSCs分化的研究。本实验拟①分离、培养、扩增胎儿Bm-MSCs,观察其生物学特性;②制备胎儿肠壁结缔组织,遴选体外培养时间;③观察胎儿肠壁结缔组织能否诱导Bm-MSCs分化形成上皮细胞?EGF在胎儿肠壁结缔组织诱导Bm-MSCs分化中的作用。为Bm-MSCs在消化管组织工程以及肠粘膜损伤性疾病治疗研究中的应用,提供基础理论和实验依据。第一部分 1材料与方法 1.1收集6例4~5个月引产胎儿的四肢骨髓,分离、培养、扩增获取Bm-MSCs。检测Bm-MSCs的贴壁率、生长曲线;流式细胞仪检测CD29、CD71、CD34及HLA-DR的表达,以及细胞周期分析;细胞化学染色检测非特异性酯酶、碱性磷酸酶及糖原。 1.2 DAPI标记Bm-MSCs,不同时点荧光显微镜观察,计算标记率。
[Abstract]:Objective Bone marrow mesenchymal stem cells (Bm-MSCs) are highly proliferative, self-renewing, and can differentiate into three layers of cells. Bm-MSCs can be widely used in many fields, such as tissue damage repair and cell transplantation therapy, etc. At present, bone marrow mesenchymal stem cells (Bm-MSCs) can differentiate into three layers of cells. Bm-MSCs was successfully induced to differentiate into osteoblasts, chondrocytes, adipocytes, nerve cells, hepatocytes, skin epithelial cells and so on. Fetal Bm-MSCswere amplified to observe the biological characteristics of fetal intestinal wall connective tissue was prepared. The selection of culture time in vitro to observe whether fetal intestinal wall connective tissue can induce Bm-MSCs differentiation into epithelial cells. The role of EGF in the differentiation of Bm-MSCs induced by connective tissue of fetal intestinal wall provides theoretical and experimental basis for the application of Bm-MSCs in the study of digestive tract tissue engineering and the treatment of intestinal mucosal injury diseases. Part one. Materials and methods. 1.1 Bm-MSCswere collected from bone marrow of 6 fetuses of 4 ~ 5 months of induced labor. The adherent rate and growth curve of Bm-MSCs were detected by Bm-MSCs.The expression of CD29, CD71, CD34 and HLA-DR, and cell cycle analysis were detected by flow cytometry. Nonspecific esterase, alkaline phosphatase and glycogen were detected by cytochemical staining. 1. 2 DAPI labeled Bm-MSCs, fluorescence microscope at different time points, the labeling rate was calculated.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321

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