人巨细胞病毒感染ECV304细胞的病变效应及基因组表达谱研究

发布时间：2018-03-19 21:35

本文选题：人巨细胞病毒　切入点：表达谱芯片　出处：《第一军医大学》2006年博士论文　论文类型：学位论文

【摘要】：人巨细胞病毒(human cytomegalovirus，HCMV)是人类最常见的先天性感染病毒，HCMV的人群感染率高达80％以上，是目前引起孕妇活动性感染及胎儿宫内感染最常见的病因之一，造成出生缺陷，与弓形虫(Toxoplasmosis)、风疹病毒(Rubella virus)、单纯疮疹病毒(Herpes simplex virus)、梅毒螺旋体(Syphilis)一起，并称为人类五大生物致畸因子。同时，HCMV也是机体免疫功能低下的人群，如艾滋病患者、放射损伤个体、器官移植和恶性肿瘤等病人并发感染死亡的常见原因之一。肾、肝、心脏、肺等器官移植后HCMV感染率分别达8％、29％、25％及39％。此外，HCMV还与心血管疾病、肿瘤的发生密切相关。因而，开展HCMV感染的病变特征与分子致病机制的研究，对于HCMV感染的防治和人口素质的提高都具有重要的意义。 HCMV即人类疱疹病毒5型(human herpes virus 5，HHV-5)，属于疱疹病毒β亚科。病毒颗粒为正二十面体，核酸外壳由一个蛋白质包膜及脂质双分子层组成，中间为病毒基质，成熟的病毒颗粒大小约200nm，其核衣壳直径大小约为100nm。内部核酸为线性双链DNA分子。HCMV基因组DNA大小为230kb，是动物DNA病毒基因组最大的一类，编码了200多种不同的病毒蛋白，由长独特序列(Unique Long，UL)和短独特序列(Unique Short，US)及两端的反向重复序列TRL、IRL、IRS及TRS组成，，通过UL和US连接部位重复序
[Abstract]:Human cytomegalovirus (HCMV) is the most common human congenital infection virus infection rate of more than 80% people, is currently one of the most common causes of maternal active infection and fetal intrauterine infection, resulting in birth defects. Together with Toxoplasma gondii, Rubella virusus, Herpes simplex virusus, Treponema pallidum and Treponema pallidum, they are also known as five major human teratogenic factors. The infection rate of HCMV after transplantation of kidney, liver, heart, lung and other organs was 82% and 39% respectively. In addition, HCMV was closely related to cardiovascular disease and tumor. It is of great significance for the prevention and treatment of HCMV infection and the improvement of population quality to study the pathological characteristics and molecular pathogenesis of HCMV infection. HCMV is human herpes virus 5 HHV-5, belonging to the subfamily 尾 of herpesvirus. The virus particles consist of 20 hedrons, and the nucleic acid shell is composed of a protein envelope and a lipid bilayer, with a virus matrix in the middle. The size of mature virus particles is about 200 nm, and the diameter of nucleocapsid is about 100 nm. The internal nucleic acid is a linear double-stranded DNA molecule. The genomic DNA size of HCMV is 230kb. it is the largest class of animal DNA virus genome and encodes more than 200 different viral proteins. It is composed of long unique sequence, short unique sequence, short unique sequence, and reverse repeat sequence TRL, IRL, IRS and TRS, which are repeated by UL and US connection site.
【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R373

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