广州市2012年腹泻病人沙门菌血清学、耐药及PFGE分型分析

发布时间：2018-03-20 03:38

本文选题：沙门菌　切入点：血清学　出处：《现代预防医学》2014年15期 　论文类型：期刊论文

【摘要】：目的研究引起患者感染性腹泻的沙门菌的血清学分布、耐药特点及分子流行病学特征。方法对分离自广州市8所哨点医院腹泻病人粪便的沙门菌,用血清凝集实验确定血清型,用K-B纸片法检测对12种抗生素的敏感性,用脉冲场凝胶电泳(PFGE)对鼠伤寒沙门菌和肠炎沙门菌进行分子分型。结果从分离的205株沙门菌中,鼠伤寒沙门菌是主要的血清型,占43.4%,其次是肠炎沙门菌,占13.7%。在抗生素敏感性测试中,磺胺复合物耐药率最高,达66.8%;其次是氨苄西林,65.4%;对头孢吡肟和头孢噻肟也出现了耐药,分别为9.8%和9.3%。PFGE分型结果显示71株鼠伤寒沙门菌可分为47个PFGE型,28株肠炎沙门菌可以分为17个PFGE型。结论本研究表明引起腹泻沙门菌血清型以鼠伤寒和肠炎为主。PFGE分型较为分散。值得注意的是,对头孢类抗生素出现较高的耐药率,应当引起重视。
[Abstract]:Objective to study the serological distribution, drug resistance and molecular epidemiology of Salmonella in patients with infectious diarrhea. Methods Salmonella isolated from feces of diarrhea patients in 8 sentinel hospitals in Guangzhou was determined by serum agglutination test. The sensitivity to 12 antibiotics was detected by K-B disk method, and the molecular typing of Salmonella typhimurium and Salmonella enteritidis was carried out by pulsed field gel electrophoresis (PFGE). Results Salmonella typhimurium was the main serotype from 205 strains of Salmonella enteritidis isolated. Accounting for 43.4, followed by Salmonella enteritidis, accounting for 13.7. in the antibiotic sensitivity test, sulfanilamide complex resistance rate was the highest (66.8%); ampicillin 65.4am; cefepime and cefotaxime resistance also appeared. The results showed that 71 strains of Salmonella typhimurium could be divided into 47 PFGE and 28 strains of Salmonella enteritidis. Conclusion this study shows that the serotypes of Salmonella diarrhea are mainly typhus and enteritis. The classification is more dispersed. It is worth noting that, The high rate of resistance to cephalosporins should be paid more attention to.
【作者单位】：广州市疾病预防控制中心;中山大学公共卫生学院;
【基金】：广州市医药卫生科技项目(2013A011090004)
【分类号】：R378.22

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