品系、性别和生物节律对小鼠旷场实验的影响

发布时间：2018-03-21 12:13

本文选题：旷场实验　切入点：探索行为　出处：《西安交通大学学报(医学版)》2014年05期 　论文类型：期刊论文

【摘要】：目的探讨性别、品系以及昼夜生物节律对旷场实验的影响。方法利用旷场实验检测不同性别的C57BL/6、BALB/c、昆明小鼠在白天与夜间对新环境的探索行为与自发活动能力。结果 3种品系小鼠旷场实验结果显示:C57BL/6在旷场中探索活动和自发活动量最低,显著低于BALB/c和昆明小鼠;C57BL/6雌、雄小鼠在白天和夜晚探索活动和自发活动量未见差异,而BALB/c和昆明小鼠分别在白天和夜间呈现性别差异;C57BL/6雌雄小鼠昼夜探索活动和自发活动量差异均无统计学意义,而BALB/c和昆明雌性小鼠呈现夜间高于白天,雄性小鼠则无此表现。结论 C57BL/6小鼠在新环境中探索活动少,自发活动量低,且受性别、节律因素影响较小,适合用于神经行为学和焦虑相关行为研究。
[Abstract]:Objective to explore gender, Methods the open field experiment was used to detect the exploring behavior and spontaneous activity of Kunming mice to the new environment during the day and night by using the open field experiment to detect the different sex of C57BL / 6 BALB / c. Results the results showed that the three strains were small. The results of rat open field experiment showed that: C57BL / 6 had the lowest amount of exploratory activity and spontaneous activity in the open field. It was significantly lower than that of C57BL / 6 females in BALB/c and Kunming mice. There was no significant difference in the amount of exploring activity and spontaneous activity between male and female mice during the day and night. However, there was no significant difference in the diurnal activity and spontaneous activity between male and female C57BL / 6 mice in BALB/c and Kunming mice during the day and night, but the BALB/c and Kunming female mice were higher in night than in daytime. Conclusion C57BL / 6 mice have less exploring activity, lower spontaneous activity and less influence by sex and rhythm factors in the new environment, so it is suitable for neurobehavioral and anxiety related behavior research.
【作者单位】：西安交通大学宗濂书院;西安交通大学启德书院;西安交通大学医学部法医学院;
【基金】：国家自然科学基金资助项目(No.81171262,81371473) 国家级大学生创新训练基金项目(No.201310698072)~~
【分类号】：R338

【相似文献】