自噬对HIV-1gp120V3环介导的海马神经元L型钙离子通道电流的影响

发布时间：2018-03-21 20:01

本文选题：自噬　切入点：HIV-　出处：《中国病理生理杂志》2014年11期 　论文类型：期刊论文

【摘要】：目的:观察哺乳动物雷帕雷素靶蛋白(m TOR)依赖性自噬信号转导通路对HIV-1 gp120V3环介导的海马神经元L-型钙离子通道电流的影响。方法:取出生1 d内的乳鼠海马神经元原代培养7 d后用于实验。实验分为2个平行实验组,即空白对照组、gp120V3组、自噬阻断剂3-甲基腺嘌呤(3-MA)组和gp120V3+3-MA组;空白对照组、gp120V3组、自噬激动剂雷帕雷素(rapamycin)组和gp120V3+rapamycin组。以膜片钳技术记录海马神经元的L型钙离子通道电流。结果:gp120V3组及3-MA组与空白对照组比较,L型钙离子通道电流密度增大(P0.05);gp120V3+3-MA组与gp120V3组比较,L-型钙离子通道电流密度增大(P0.05);rapamycin组与空白对照组比较,L型钙离子通道电流密度减小(P0.05);gp120V3+rapamycin组与gp120V3组比较,L型钙离子通道电流密度减小(P0.05)。结论:从电生理角度证明,自噬可能参与了HIV-1gp120V3环介导的海马神经元L型钙离子通道电流的改变。
[Abstract]:Aim: to observe the effect of mammalian Raparex target protein m torque on L-type calcium channel currents in hippocampal neurons mediated by HIV-1 gp120V3 ring. Methods: hippocampal neurons of newborn rats were taken out within 1 day after birth. The primary culture was used for 7 days. The experiment was divided into two parallel experimental groups. That is, blank control group gp120V3, autophagy blocker 3-methyladenine 3-MA-group and gp120V3 3-MA group, blank control group gp120V3 group, The L-type calcium channel currents of hippocampal neurons were recorded by patch clamp technique in the autophagy agonist rapamycinin group and gp120V3 rapamycin group. Results compared with the control group, the L-type calcium channel current density increased in the 3-MA group and the control group (P0.05Gp120V3 3-MA). Compared with gp120V3 group and gp120V3 group, the current density of L-type calcium channel increased in P0.05 and rapamycin group, and the decrease in current density of L-type calcium channel in P0.05Gp120V3 rapamycin group compared with that in gp120V3 group. Conclusion: from the electrophysiological point of view, the current density of L-type calcium channel decreases. Autophagy may be involved in the changes of L-type calcium channel currents in hippocampal neurons mediated by HIV-1gp120V3 ring.
【作者单位】：广州市番禺区中心医院;暨南大学医学院病理生理学教研室;暨南大学医学院国家中医药管理局病理生理学三级科研实验室;
【基金】：国家自然科学基金资助项目(No.8117134;No.81471235) 广东省自然科学基金资助项目(No.9151040701000) 广东省科技计划资助项目(No.2010B030700016) 广州市科技计划资助项目(No.2010Y1-C291)
【分类号】：R338.8

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