晚期糖基化终产物改变心肌细胞自发钙火花形态学

发布时间：2018-03-24 08:45

本文选题：晚期糖基化终产物　切入点：心肌细胞　出处：《中国病理生理杂志》2014年11期

【摘要】：目的:本研究旨在探讨晚期糖基化终末产物(AGEs)对心肌细胞雷诺丁受体(RyR)活性的影响及钙调控作用,从而阐明糖尿病心肌病心脏功能障碍的潜在机制。方法:通过激光共聚焦显微成像技术在培养的乳鼠心肌细胞观察自发钙火花发放频率以及钙火花的形态。结果:与对照组相比,AGEs大幅度增加钙火花的发放频率,其中10 mg/L AGEs组增加48%(P0.01),50 mg/L组AGEs增加200%(P0.01);而150 mg/L组AGEs组增加110%(P0.01)。AGEs改变自发钙火花的形态、降低钙火花的幅度、宽度和钙火花持续时间并增加心肌细胞静息期钙离子水平。结论:AGEs引起自发钙火花的发放频率,从而增强钙火花介导的肌浆网Ca2+漏流,增加心肌细胞静息期钙离子水平,这可能是引起糖尿病性心肌病收缩功能障碍的重要原因。
[Abstract]:Aim: to investigate the effects of advanced glycation end product (ages) on the activity of renotin receptor RyR (RyR) and the regulation of calcium in cardiac myocytes. Methods: the frequency of spontaneous calcium sparks and the morphology of calcium sparks were observed in cultured neonatal rat cardiomyocytes by confocal laser microscopy. Compared with the control group, ages significantly increased the discharge frequency of calcium sparks. In the 10 mg/L AGEs group, the AGEs of the 10 mg/L AGEs group was increased by 48% and the AGEs of the 50 mg/L group increased by 200kW P0.01C, while the AGEs group of 150 mg/L group increased 110%(P0.01).AGEs to change the morphology of spontaneous calcium sparks and to decrease the amplitude of calcium sparks. The width and duration of calcium sparks and the level of Ca ~ (2 +) in cardiomyocytes during resting period were increased. Conclusion the spontaneous calcium sparks were induced by the calcium sparks, which enhanced the leakage of sarcoplasmic reticulum (Ca2) induced by calcium sparks, and increased the calcium level of cardiac myocytes during the resting period. This may be an important cause of systolic dysfunction in diabetic cardiomyopathy.
【作者单位】：深圳大学第一附属医院内分泌科;南方医科大学病理生理学教研室;深圳大学医学院病理生理学教研室;
【基金】：国家自然科学基金资助项目(No.81200122)
【分类号】：R363

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