水疱性口炎病毒反向遗传重组载体的构建

发布时间：2018-03-30 05:19

本文选题：水疱性口炎病毒　切入点：反向遗传　出处：《吉林大学》2006年博士论文

【摘要】：随着RNA病毒反向遗传技术的进展,水疱性口炎病毒(vesivular stomatitisvirus,VSV)载体已被开发成为一种有效的治疗制剂。VSV病毒载体是一种高效的溶瘤病毒载体,具有非常广的溶瘤范围。资料报道VSV载体几乎能够溶解所有的瘤细胞,在体外实验中VSV载体的溶瘤率都在50%以上,在体内实验中VSV载体能够显著延长荷瘤动物模型的寿命。VSV载体也被开发成为一种有效的疫苗载体, VSV作为疫苗载体应用到获得性免疫综合症病毒、流感病毒、丙型肝炎病毒和乙肝病毒等疫苗的研制过程中。因此水疱性口炎病毒载体具有非常好的应用前景。本实验克隆了水疱性口炎病毒的全长cDNA序列(11161bp)以及L蛋白基因序列(6330bp)、N蛋白基因序列(1269bp)和P蛋白基因序列(798bp),并构建了水疱性口炎病毒的反向遗传重组系统,即:包含VSV全长cDNA序列的反向遗传重组载体和L蛋白、N蛋白和P蛋白的表达载体,并将这四种质粒共同转染BHK-21细胞,对VSV的反向遗传重组进行探索。这为水疱性口炎病毒载体的进一步应用打下基础。
[Abstract]:With the development of reverse genetic technology of RNA virus, vesicular stomatitis virus (VSVV) vector has been developed as an effective therapeutic agent. It is reported that VSV vector can dissolve almost all tumor cells in vitro, and the rate of VSV vector is more than 50% in vitro. In vivo experiments, VSV vectors can significantly prolong the life span of tumor-bearing animal models. VSV vectors have also been developed as an effective vaccine vector. VSV is used as a vaccine vector for acquired immune syndrome virus, influenza virus. In the development of hepatitis C virus and hepatitis B virus vaccine, the vesicular stomatitis virus vector has a very good application prospect. In this study, we cloned the full-length cDNA sequence of vesicular stomatitis virus (BVV) 11161bp), the L protein gene sequence (63330bpHN) and the sequence of P protein gene (798 BP), and constructed the reverse genetic recombination system of blistering stomatitis virus (BMDV). That is, the reverse genetic recombinant vector containing the full-length cDNA sequence of VSV and the expression vector of L protein N protein and P protein were co-transfected into BHK-21 cells. The reverse genetic recombination of VSV was explored, which laid a foundation for the further application of vesicular stomatitis virus vector.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：Q78;R392

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