低氧复合运动抑制低氧诱导的骨骼肌线粒体DNA氧化损伤

发布时间：2018-03-30 05:46

本文选题：低氧复合运动　切入点：线粒体DNA氧化　出处：《生理学报》2014年05期

【摘要】：本文旨在观察低氧复合运动对低氧状态下大鼠骨骼肌线粒体DNA(mtDNA)氧化损伤及线粒体8-氧鸟嘌呤DNA糖基化酶(OGG1)表达的影响,并探讨其可能机制。雄性Sprague-Dawley(SD)大鼠随机分为常氧对照组(NC)、常氧运动组(NT)、低氧对照组(HC)和低氧复合运动组(HT)。低氧干预为常压低氧帐篷,11.3%氧浓度持续暴露4周。运动干预为跑台训练(5o,15m/min),60 min/d,5 d/周,共4周。结果显示,HC组与NC组比较,线粒体复合体I、II、IV、ATP合成酶活性和膜电位显著降低(P0.05或P0.01),锰超氧化物歧化酶(MnSOD)、谷胱甘肽过氧化物酶(GPx)和OGG1活性显著降低(P0.05或P0.01),线粒体活性氧(ROS)生成速率和mtDNA中8-oxodG含量显著升高(P0.01),SIRT3蛋白表达、骨骼肌和线粒体烟碱胺腺嘌呤二核苷酸氧化还原型比值([NAD+]/[NADH])显著降低(P0.05或P0.01)。HT组和HC组比较,线粒体复合体I、II、IV、ATP合成酶活性和膜电位显著升高(P0.05或P0.01),MnSOD、GPx、OGG1活性和线粒体OGG1蛋白表达显著升高(P0.01),线粒体ROS生成速率和mtDNA中8-oxodG含量显著降低(P0.01),SIRT3蛋白表达、骨骼肌和线粒体[NAD+]/[NADH]显著升高(P0.05或P0.01)。以上结果提示,低氧复合运动可上调线粒体OGG1和抗氧化酶,抑制低氧诱导的mtDNA氧化损伤,运动训练对[NAD+]/[NADH]和SIRT3的上调可能参与了对骨骼肌线粒体低氧耐受能力的增强调控。
[Abstract]:The purpose of this study was to observe the effects of hypoxia combined exercise on oxidative damage of mitochondrial DNA mtDNA and the expression of mitochondrial 8-oxyguanine DNA glycosylase (OGG1) in skeletal muscle of rats under hypoxia. Male Sprague-Dawley SD rats were randomly divided into normoxic control group, normoxic exercise group, hypoxic control group and hypoxic combined exercise group. In order to train for the running station, the training will be 5o / 15m / min / min / min / min / min / min / min / min / min / min / min / min / week / week. The results showed that the HC group was compared with the NC group. The activity of ATP synthase and membrane potential of mitochondrial complex I _ (2) I _ (2) O _ (1) and membrane potential decreased significantly (P0.05 or P0.01), and the activities of manganese superoxide dismutase (MnSOD), glutathione peroxidase (GPX), glutathione peroxidase (Glutathione peroxidase) and OGG1 decreased significantly (P0.05 or P0.01), the production rate of mitochondrial reactive oxygen species (Ros) and the content of 8-oxodG in mtDNA were significantly decreased. The expression of SIRT3 protein was significantly increased, The ratio of oxidative reduction of adenine dinucleotide ([NAD] / [NADH]) in skeletal muscle and mitochondria was significantly lower than that in P0.01).HT group and HC group. The activity of ATP synthase and the membrane potential of the mitochondrial complex Igni II / IVP were significantly increased (P0.05) or the activity of MNSODX OGG1 and the expression of mitochondrial OGG1 protein were significantly increased (P 0.01). The production rate of mitochondrial ROS and the content of 8-oxodG in mtDNA significantly decreased the expression of P0.01 and SIRT3 protein. [NAD] / [NADH] of skeletal muscle and mitochondria increased significantly (P0.05 or P0.01). The results suggested that hypoxia combined exercise could up-regulate mitochondrial OGG1 and antioxidant oxidase and inhibit oxidative damage of mtDNA induced by hypoxia. The up-regulation of [NAD] / [NADH] and SIRT3 in exercise training may be involved in the regulation of mitochondrial hypoxia tolerance in skeletal muscle.
【作者单位】：天津武警后勤学院军事训练医学教研室;天津武警后勤学院天津市心血管重塑与靶器官损伤重点实验室;天津武警后勤学院药理学教研室;武警西藏总队后勤部卫生处;
【基金】：supported by the grants from the National Natural Science Foundation of China(No.31200894) the Application Foundation and Cutting-edge Technology Research Project of Tianjin Municipality,China(No.12JCQNJC07900)
【分类号】：R337

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