原癌基因c-erbB2在表皮生长因子调控小鼠卵母细胞体外成熟中的作用

发布时间：2018-03-30 17:26

  本文选题：c-erbB_2　切入点：卵母细胞　出处：《江西医学院》2005年硕士论文

【摘要】：目的和方法:卵母细胞成熟的机理尚不明了。本研究建立小鼠卵母细胞体外培养模型,用不同浓度反义c-erbB_2寡脱氧核苷酸(c-erbB_2ASODN)与未成熟卵母细胞共孵育,观察其对卵母细胞体外成熟的影响;观察表皮生长因子(EGF)对卵母细胞体外成熟的作用以及c-erbB_2 ASODN 对EGF 促卵母细胞成熟的影响;利用RT-PCR 技术检测卵母细胞中c-erbB_2mRNA 表达及在EGF、c-erbB_2ASODN 等不同处理因素作用下卵母细胞成熟过程中c-erbB_2 mRNA 表达的变化。为进一步了解卵子的发育、成熟机理及其调控增添新资料,为开辟新的促生育和抗生育途径提供实验依据。 结果:(1)c-erbB_2ASODN 能呈剂量依赖方式抑制卵母细胞的GVBD 率和PBⅠ率,并显著延迟其成熟的时间(低、中、高c-erbB_2ASODN 组24hGVBD 率比对照组分别低8.9%(P0.05)、26.6%(P0.01)和37.0%(P0.01);中、高c-erbB_2ASODN 组PBⅠ率比对照组分别低13.1%(P0.05)和22.7%(P0.01))。(2)EGF 可直接刺激裸卵的成熟分裂,并与其剂量和作用时间相关(1ng/mlEGF 组、10ng/mlEGF 组和50ng/mlEGF 组在24h 时的GVBD率比对照组分别高19.0%(P0.05)、27.0%(P0.01)20.6%(P0.05), PBⅠ率比对照组分别高13.0%、21.9%和14.4%(P0.05))。(3)c-erbB_2ASODN能抑制EGF 对卵母细胞的促成熟作用(EGF+ c-erbB_2ASON 组与EGF 组相比
[Abstract]:Objective and methods: the mechanism of oocyte maturation is not clear. In this study, mouse oocytes were cultured in vitro with different concentrations of antisense c-erbB_2 oligodeoxynucleotides c-erbB 2ASODN and immature oocytes. The effect of EGF on oocyte maturation in vitro, the effect of EGF on oocyte maturation in vitro and the effect of c-erbB_2 ASODN on oocyte maturation induced by EGF were observed. RT-PCR technique was used to detect the expression of c-erbB_2mRNA in oocytes and the changes of c-erbB_2 mRNA expression during oocyte maturation under the action of EGFC-erbB2ASODN, etc. In order to further understand the oocyte development, the mechanism of maturation and its regulation, new information was added. In order to open up a new procreation and antifertility pathway to provide experimental basis. Results c-erbB2ASODN inhibited the GVBD rate and PB 鈪，

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