ndrg2在人胎脑发育中的表达特点

发布时间：2018-03-30 17:58

本文选题：ndrg2　切入点：人胎脑　出处：《第四军医大学》2006年硕士论文

【摘要】：[研究背景]ndrg2与ndrg1、ndrg3和ndrg4共同构成了一个新的基因家族，即ndrg家族。该基因家族参与了细胞的生长，但具体机制仍不清楚。人ndrg2、鼠ndrg2分别由我导师邓艳春教授和日本研究小组发现，氨基酸序列分析显示，，人ndrg2、鼠ndrg2的同源性高达95％。人ndrg2基因的基因组全长为9.33kb，位于染色体的14q11.2，mRNA全长2024bp编码含357个氨基酸残基、分子量约为40.79kD的蛋白质。曾命名为p40。同源性分析表明，其与ndrg1(也称做脑发育相关蛋白)具有高度同源性，并含有一个酰基携带蛋白样结构域，故称为ndrg2。研究结果初步显示，ndrg2在多种正常组织中高表达，而在某些肿瘤组织和肿瘤细胞系中低表达或无表达，在体外可抑制多种肿瘤细胞系的生长(如胶质瘤细胞系BT 325)，推测该基因可能与肿瘤的发生发展有关，因此提出ndrg2可能为新的候选抑癌基因。新近资料表明，ndrg2参与了抑郁症和阿尔采默病等脑部疾病的发生发展，但这个基因与脑功能及神经元发育、成熟有何关系，目前尚不清楚。[研究目的]该实验旨在系统分析ndrg2在胎脑发育不同阶段表达分布的时空特点，以期弄清ndrg2在胎脑各脑功能区的表达特点及在胎脑成熟过程中的表达趋势，为进一步研究其功能提供实验依据。[研究方法]1．用免疫组化染色方法从蛋白质水平确定ndrg2在16～28周胎脑各脑功能区的时空表达特点及表达趋势。用抗ndrg2单克隆抗体，对16～28周各月份各脑功能区石蜡切片进
[Abstract]:Background ndrg2, together with ndrg1ndrg3 and ndrg4, constitute a new gene family, ndrg family.The gene family is involved in cell growth, but the exact mechanism remains unclear.The amino acid sequence analysis of human ndrg2 and mouse ndrg2 showed that the homology of human ndrg2 and mouse ndrg2 was 95%.The full length of human ndrg2 gene is 9.33 kb. The full-length 2024bp of 14q11.2mRNAs encodes a protein with a molecular weight of about 40.79kD containing 357 amino acid residues.Once named p40.Homology analysis showed that it had high homology with ndrg1 (also known as brain development-related protein) and contained an acyl-carrying protein-like domain, so it was called ndrg2.The results showed that the expression of ndrg2 was high in many normal tissues, but low or no expression in some tumor tissues and tumor cell lines.Recent data indicate that ndrg2 is involved in the development of brain diseases such as depression and Alzheimer's disease, but it is not clear how this gene is related to brain function and neuronal development and maturation.[objective] to systematically analyze the temporal and spatial characteristics of expression and distribution of ndrg2 in different stages of fetal brain development in order to find out the expression characteristics of ndrg2 in various functional areas of fetal brain and its expression trend during fetal brain maturation.To provide experimental basis for further study of its function.[research methods] 1.Immunohistochemical staining was used to determine the temporal and spatial expression characteristics and expression trend of ndrg2 in various functional brain regions of the fetal brain at 1628 weeks.Using anti ndrg2 monoclonal antibody, paraffin sections of the functional regions of the brain in each month of 16 weeks 28 weeks were embedded in paraffin sections.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321

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