低分子量硫酸葡聚糖抑制异种移植超急性排斥反应的研究

发布时间：2018-03-31 17:52

本文选题：硫酸葡聚糖　切入点：猪　出处：《华中科技大学》2007年硕士论文

【摘要】： 第一部分低分子量硫酸葡聚糖抑制人补体系统活性的研究目的研究不同浓度分子量为5000的低分子量硫酸葡聚糖(DXS)对人补系统活性的抑制作用。方法根据DXS的浓度不同分为5个实验组,DXS浓度分别为0.1mg/ml, 0.5mg/ml, 1mg/ml, 2mg/ml和4mg/ml,不含DXS组作为对照。CH50法检测含有不同终浓度DXS的人血清的补体活性,MTT比色法测定含不同终浓度DXS的人血清对猪内皮细胞杀伤率,免疫荧光法检测猪内皮细胞表面补体C3和IgM、IgG抗体沉积。结果DXS可以抑制人血清补体活性,抑制人血清对猪内皮细胞的毒性作用,剂量越大,抑制率越高,细胞表面补体C3抗体沉积越少,IgM、IgG抗体沉积没有明显差异。结论低分子量硫酸葡聚糖能有效抑制人补体系统活性第二部分低分子量硫酸葡聚糖抑制人补体介导的异种移植超急性排斥反应的研究目的研究分子量为5000的低分子量硫酸葡聚糖(DXS)抑制人补体激活而抑制异种抑制超急性排斥反应的发生。方法建立balbc小鼠心脏体外灌注模型,用含DXS终浓度分别为0.1mg/ml(n=8),2.0mg/ml(n=9)的10%人血清灌注液进行灌注,以不含DXS的人血清组为对照(n=7),研究低分子量硫酸葡聚糖抑制人补体激活从而抑制人补体介导的超急性排斥反应. 结果心脏搏动时间对照组(n=7),0.1mg/ml组(n=7)和2.0mg/ml组(n=7)分别为17±11min, 49±15min和123±19min,含DXS组心脏搏动时间明显长于对照组(Dunnett's T检验,P0.01);免疫荧光及免疫酶染色显示2.0mg/ml组心脏内补体C3c,C5b-9沉积较对照组明显减少。结论低分子量硫酸葡聚糖能有效抑制人补体激活从而抑制补体介导的超急性排斥反应发生。
[Abstract]:A study on the inhibition of human complement system activity by low molecular weight dextran sulfate. Objective to study the inhibitory effect of low molecular weight dextran sulfate (DXS) with different concentration of 5000 on the activity of human supplement system. Methods according to the concentration of DXS, five experimental groups were divided into five groups: 0.1 mg / ml, 0.5 mg / ml, 1 mg / ml, 1 mg / ml, 2mg/ml and 4 mg / ml, respectively. The complement activity of human serum containing different final concentrations of DXS was determined by colorimetric method without DXS. The killing rate of human serum of DXS on porcine endothelial cells, The antibody deposition of complement C 3 and IgG on the surface of porcine endothelial cells was detected by immunofluorescence method. Results DXS could inhibit the complement activity of human serum and the toxicity of human serum to porcine endothelial cells. The higher the dose, the higher the inhibition rate, and the less C3 antibody deposition on the cell surface had no significant difference. Conclusion low molecular weight dextran sulfate can effectively inhibit the activity of human complement system. The second part of the study on suppressive effect of low molecular weight dextran sulfate on human complement mediated xenograft hyperacute rejection. Objective to study the effect of low molecular weight dextran sulfate (DXS) with molecular weight of 5000 on human complement activation and xenogeneic inhibition of hyperacute rejection. Methods the heart of balbc mice was perfused in vitro with 10% human serum perfused with 0.1 mg / ml of DXS final concentration of 2. 0 mg / ml of DXS. Low molecular weight dextran sulfate inhibited the activation of human complement and inhibited human complement mediated hyperacute rejection. Results the cardiac beating time in the control group was 17 卤11 min, 49 卤15min and 123 卤19 min in the 2.0mg/ml group and 0.1 mg / ml group, respectively. The cardiac beating time in the DXS group was significantly longer than that in the control group (P 0.01), and immunofluorescence and immunoenzyme staining showed that the complement C5b-9 deposition in the heart of the 2.0mg/ml group was significantly longer than that in the 2.0mg/ml group. The control group was significantly decreased. Conclusion low molecular weight dextran sulfate can effectively inhibit the activation of human complement and inhibit the development of complement mediated hyperacute rejection.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R96;R392

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