双歧杆菌细胞壁的免疫调节和抑瘤作用研究

发布时间：2018-03-31 23:34

本文选题：青春双歧杆菌细胞壁　切入点：巨噬细胞　出处：《安徽理工大学》2006年硕士论文

【摘要】：目的探讨青春双歧杆菌细胞壁对小鼠的免疫调节作用及其对人移行细胞膀胱癌细胞的体内外抑制作用。方法采用青春双歧杆菌,提取其细胞壁成分并腹腔注射于KM小鼠,连续7天,处死小鼠,无菌收集腹腔巨噬细胞,以ELISA法测定腹腔巨噬细胞产生的细胞因子IL-1、IL-12及TNF-α的含量。采用细胞人移行细胞膀胱癌细胞(T_(24))传代培养至对数生长期,小鼠皮下接种,建立膀胱癌小鼠模型,腹腔注射双歧杆菌细胞壁,2周后,剥离瘤体,称瘤重,计算抑瘤率:MTT法体外测定双歧杆菌细胞壁对T_(24)细胞的抑制作用;无菌收集腹腔巨噬细胞,以ELISA法测定腹腔巨噬细胞产生的细胞因子IL-1、IL-12及TNF-α的含量。结果青春双歧杆菌细胞壁注射组腹腔巨噬细胞产生的IL-1的A值为0.62±0.04,对照组为0.34±0.02,IL-12的含量为(823.34±185.32)pg/ml,而对照组则为(498.26±105.43)pg/ml及TNF-α的含量为(1.32±4.25)ng/ml对照组为(0.45±0.33)ng/ml,分别明显高于对照组,两者比较具有非常显著性差异(P0.01);建立膀胱癌小鼠模型,接种后第1周左右局部可触及肿块,第2周局部肉眼可见肿块,BCW注射组可以使小鼠局部肿瘤体积减小,肿瘤重量减轻与对照组之间比较有非常显著性差异(P0.01);不同浓度双歧杆菌细胞壁在体外均能抑制T_(24)细胞生长,且浓度越大,时间越长,对该细胞的抑制作用越强(P0.05);IL-1的A值为0.76±0.02,对照组为0.41±0.05,IL-12的含量为(786.34±154.32)pg/ml,而对照组则为(520.35±89.37)pg/ml及TNF-α的含量为(1.41±4.25)ng/ml对照组为(0.52±0.18)ng/ml,分别明显高于对照组,两者比较具有非常显著性差异(P0.01)。结论青春双歧杆菌细胞壁成分能激活小鼠腹腔巨噬细胞,使之分泌多量的IL-l、IL-12及TNF-α,且在体内外均能抑制人移行细胞膀胱癌细胞的生长。
[Abstract]:Objective to investigate the immunomodulatory effect of bifidobacterium pubertifolia cell wall in mice and its inhibitory effect on human transitional cell bladder cancer cells in vitro and in vivo. Methods Bifidobacterium pubertii was used to extract the cell wall components and injected intraperitoneally into km mice. The mice were killed for 7 days and the peritoneal macrophages were collected aseptically. The levels of cytokines IL-1mIL-12 and TNF- 伪 produced by peritoneal macrophages were determined by ELISA method. The mice were inoculated subcutaneously to establish the bladder cancer model by subcutaneously inoculating the cytokines IL-1- IL-12 and TNF- 伪. After 2 weeks of intraperitoneal injection of bifidobacterium cell wall, the tumor was stripped and weighed. The inhibition rate of bifidobacterium cell wall on Tast24) cells was measured by the cell wall of bifidobacterium in vitro, and the peritoneal macrophages were collected aseptically. The levels of cytokines IL-1- (IL-12) and TNF- 伪 (TNF- 伪) produced by peritoneal macrophages were determined by ELISA method. Results the A value of IL-1 produced by peritoneal macrophages was 0.62 卤0.04 in the cell wall injection group of Bifidobacterium pubescens, 0.34 卤0.02 渭 g / ml in the control group, 823.34 卤185.32 ng / ml in the control group, and 0.45 卤0.33 ng / ml in the control group (P < 0.01). There was a very significant difference between the two groups (P 0.01). The tumor could be reached around 1 week after inoculation, and the local tumor volume could be reduced in the BCW injection group at the second week. Compared with the control group, there was a significant difference between the tumor weight reduction and the control group. The cell wall of Bifidobacterium at different concentrations could inhibit the growth of TST-24 cells in vitro, and the higher the concentration was, the longer the time was. The stronger the inhibitory effect on the cells was, the higher the A value of IL-1 was 0.76 卤0.02, the content of IL-12 was 786.34 卤154.32 渭 g / ml in the control group, and the content of TNF- 伪 was 0.52 卤0.18 ng / ml in the control group, which was significantly higher than that in the control group (P 0.01). Conclusion the cell wall components of Bifidobacterium pubertifolia can activate mouse peritoneal macrophages and secrete a large amount of IL-lt- 12 and TNF- 伪, and inhibit the growth of human transitional cell bladder cancer cells in vivo and in vitro.
【学位授予单位】：安徽理工大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R371

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