性激素对卵巢囊淋巴孔调控和卵巢囊淋巴孔的发育学研究

发布时间：2018-04-01 00:13

本文选题：淋巴孔　切入点：激素　出处：《浙江大学》2006年硕士论文

【摘要】：研究背景卵巢囊是由联系卵巢和阔韧带的腹膜高度发育而成的一个包裹卵巢的囊样结构,它与输卵管远端相连,形成一个完全闭合的囊腔,称为卵巢囊腔(ovarian bursal cavity),囊腔内含有液体(bursal fluid)。卵巢囊腔为卵子正常生长发育提供了一个相对稳定的体液环境。Shinohara首先描述了金仓鼠的卵巢囊内壁上皮存在淋巴孔,并提出这些淋巴孔具有物质转运功能,可能影响卵巢发育和生殖。随后,在欧洲海狸、豚鼠卵巢囊上皮以及人卵巢上皮都发现淋巴孔。但卵巢囊淋巴孔的研究多集中在形态结构观察,对其发育学和功能研究尤其是性激素对淋巴孔的干预,尚鲜见报道。研究目的 (1) 研究卵巢囊淋巴孔的超微结构以揭示其作为卵巢囊腔内物质引流通路的结构基础,探讨卵巢与卵巢囊之间的相互作用,阐明卵巢囊及其淋巴孔具有重要的生殖意义。(2) 观察性激素干预对小鼠卵巢囊淋巴孔的调控作用及对淋巴吸收的影响,并对其机制进行了初步探讨。(3) 对胚胎豚鼠和出生后各个时期的豚鼠卵巢囊淋巴孔进行研究,为进一步开展卵巢囊淋巴孔的功能研究提供发育学资料。研究方法 (1) 通过光镜、常规扫描电镜、NaOH消化法和透射电镜观察小鼠卵巢囊及卵巢囊淋巴孔。(2) 性激素干预对小鼠卵巢囊淋巴孔的调控及对示踪剂吸收的影响,并用Elescope计算机图像处理软件对卵巢囊淋巴孔作定量处理。(3)
[Abstract]:Background Ovarian sac is a highly developed peritoneum associated with the ovary and the broad ligament. It is a cystic structure surrounding the ovary. It is connected to the distal end of the fallopian tube to form a completely closed cystic cavity.It is called ovarian bursal cavity.It contains liquid bursal fluid.The ovarian cavity provides a relatively stable humoral environment for the normal growth and development of the egg. Shinohara first describes the existence of lymphatic pores in the inner wall of the ovarian cyst of the hamster, and suggests that these lymphatic pores have the function of substance transport.May affect ovarian development and reproduction.Subsequently, lymphatic foramen were found in European beavers, guinea pig ovarian sac epithelium and human ovarian epithelium.However, the study of ovarian cystic lymphatic foramen is mainly focused on morphological and structural observation. There are few reports on the development and function of ovarian cystic lymphatic foramen, especially on the intervention of sex hormone on lymphatic foramen.Objective 1) to study the ultrastructure of lymphatic foramen of ovarian cysts in order to reveal its structural basis as a pathway of material drainage in ovarian cyst, and to explore the interaction between ovary and ovarian capsule.To elucidate the important reproductive significance of ovarian sac and its lymphatic foramen. (2) to observe the effect of sex hormone intervention on the regulation of lymphatic foramen of ovarian sac in mice and its effect on lymphatic absorption.The mechanism of ovarian cystic lymphatic foramen in embryonic guinea pigs and postnatal guinea pigs was studied, which provided developmental data for further study on the function of ovarian cystic lymphatic foramen.Methods 1) the effects of sex hormone intervention on the regulation of ovarian sac and lymphatic foramen of ovary and the absorption of tracer in mice were observed by light microscope, routine scanning electron microscopy (SEM) and NaOH digestion and transmission electron microscopy (TEM).Quantitative analysis of ovarian cystic lymphatic foramen with Elescope image processing software
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

【参考文献】

中国期刊全文数据库前10条

1 李继承,沈毅,Bruno TOTA,Giovanni ZUMMO;AngⅡ诱导的VEGF对食用蛙心包淋巴孔和间皮血窦的影响[J];动物学报;2002年03期

2 李燕园,李继承;家兔肋胸膜淋巴孔的发现[J];动物学报;2002年06期

3 张宁,李继承;小鼠卵巢囊淋巴孔的超微结构及其吸收功能[J];动物学报;2005年01期

4 隋梅花,李继承;人类卵巢淋巴孔的发现及其意义[J];解剖学报;2001年03期

5 李继承，赵章仁，陈汉民，周吉林，叶学渊，俞寿民;人与小鼠膈腹膜乳斑的研究[J];解剖学报;1996年01期

6 李燕园,李继承;腹膜淋巴孔与淋巴转归NO-cGMP-Ca~(2+)信号转导途径研究[J];生理学报;2005年01期

7 隋梅花,李继承;豚鼠卵巢囊淋巴孔的发现及卵巢囊超微结构研究[J];实验生物学报;2001年04期

8 隋梅花,李继承;妊娠和激素干预对豚鼠卵巢囊淋巴孔的影响[J];实验生物学报;2003年01期

9 李继承,杨泽然,Bruno Tota;NO对小鼠腹膜淋巴孔的调控作用与腹膜透析失超滤机理研究[J];中国病理生理杂志;2002年09期

10 李继承，，吕志连，石元和，沈毅，陈一芳;腹膜孔的药物调节和计算机图象处理[J];中国医学科学院学报;1996年03期