碘过量对大鼠仔鼠垂体TSH细胞和胸腺的形态学影响

发布时间：2018-04-05 15:39

本文选题：碘过量　切入点：TSH细胞　出处：《天津医科大学》2006年硕士论文

【摘要】：目的观察不同浓度碘酸钾喂养的20日龄和60日龄Wistar大鼠仔鼠其垂体TSH细胞的形态学变化,进一步探讨碘过量对垂体-甲状腺轴影响的机制。方法选用断乳后一个月,体重在120-140g的Wistar大鼠,雌雄各半,随机分为6组:①低碘组(LI);②适碘组(NI);③5倍碘组(5HI);④10倍碘组(10HI);⑤50倍碘组(50HI);⑥100倍碘组(100HI)。LI组动物饲以低碘地区的粮食(玉米、小麦、黄豆)制成的低碘饲料并添加动物必需的无机盐和维生素,平均碘含量小于50μg/kg,其它5组饲以正常鼠料(平均碘含量300μg/kg),上述两种饲料中各种粮食的配比是相同的,以保证饲料的主要营养素相同,只是碘含量不同。LI组饮用去离子水,NI组饮用自来水,5HI、10HI、50HI、100HI组大鼠分别饮用含不同浓度的碘酸钾的自来水,其饮水中碘含量(以I-计)分别为820μg/L、1845μg/L、10045μg/L和20295μg/L。大鼠每日碘摄入量估计如下:大鼠每日进食20g,进水30mL,天津市自来水的碘含量为5μg/L,去离子水碘含量为0μg/L,各组动物每日摄碘量估计为1μg/L(LI),6.15μg/L(NI),30.75μg/L(5HI),61.5μg/L(10HI),307.5μg/L(50HI),615.0μg/L(100HI)。喂养3个月后,雌雄大鼠按1∶1比例合笼交配生育仔鼠。断乳后各组仔鼠的喂养方法同前。观察20日龄、60日龄仔鼠垂体TSH细胞的组织学变化,测定20日龄、60日龄仔鼠垂体和60日龄甲状腺的相对重量,对垂体TSH细胞的体密度和面数密度进行形态计量学分析,并计数分析60日龄仔鼠TSH的强阳性细胞数。结果 (1)20日龄、60日龄LI组和各碘过量组仔鼠垂体的相对重量与NI组相
[Abstract]:Objective to observe the morphological changes of pituitary TSH cells in 20-day-old and 60-day-old Wistar rats fed with different concentrations of potassium iodate, and to explore the mechanism of the effect of iodine excess on pituitary-thyroid axis.Methods one month after weaning, Wistar rats, weighing 120-140 g, were divided into two groups: male and female.The low iodine diet made of soybeans and added inorganic salts and vitamins necessary for animals, the average iodine content was less than 50 渭 g / kg, and the other five groups were fed with normal diet (average iodine content was 300 渭 g / kg 路kg ~ (-1)), and the proportion of all kinds of food in the above two kinds of feed was the same.After feeding for 3 months, male and female rats were mated at 1:1.After weaning, the feeding methods of each group were the same as before.To observe the histological changes of pituitary TSH cells in 20-day and 60-day-old rats, to measure the relative weight of pituitary gland and thyroid gland of 20-day-old and 60-day-old rats, and to analyze the volume density and surface number density of pituitary TSH cells by morphometry.The number of strong positive cells of TSH in 60-day-old rats was counted and analyzed.Results the relative weight of pituitary gland of 20 days old and 60 days old Li group and each iodine excess group was compared with that of NI group.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R361

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