L-精氨酸诱导大鼠暴发性胰腺炎模型的建立

发布时间：2018-04-08 07:41

本文选题：L-精氨酸　切入点：暴发性胰腺炎　出处：《汕头大学》2006年硕士论文

【摘要】：背景目的：暴发性胰腺炎(fulminant acute pancreatitis，FAP)作为一种特殊类型的胰腺炎(acute pancreatitis，AP)，其临床特点是发病迅速、来势凶险、早期即可出现多器官功能不全、死亡率高。虽然目前临床上常有这类胰腺炎病因、治疗等方面的报道，但国内外对FAP以及胰外器官损伤情况研究极少，认识也极其有限。因此，建立FAP动物模型，进而对FAP以及胰外器官损伤情况进行系统性研究，降低临床FAP死亡率，有极其重要的意义。本实验拟用L-精氨酸(L-arginine，L-Arg)作为AP诱导剂，通过新的注射方式来探讨大鼠FAP模型的建立。方法：144只雄性Wistar大鼠，随机分为实验组(L-Arg组，n=72)和对照组(NS组，n=72)。L-Arg组：20％L-精氨酸先从腹腔内注射(450mg／100g大鼠体重)一次，间隔2h后，再从皮下注射(150mg／100g大鼠体重)一次。NS组：以同样的方式注射等体积的生理盐水。两组于末次注射后各取30只大鼠，观察0h—120h内大鼠病死率；在0、6、12、24、48、72和120h各相应时点各取6只大鼠处死，观察大鼠血清淀粉酶、脂肪酶、胰腺湿／干比率、血常规、肝功能、肾功能、肺功能的变化；应用HE染色观察胰腺、肝脏、肾脏、肺脏在以上各时点的病理改变，并给予病理评分或评级。结果：L-Arg组和NS组分别经两次间隔2h于腹腔和皮下注射药物后： (1)L-Arg组30只大鼠，0h～6h死亡0只，6～12h死亡1只，12～24h死亡2只，24～48h死亡7只，48～72h死亡5只，72～120h死亡1只，总死亡率为16／30=53.33％。NS组30只大鼠120h内无死亡。 (2)L-Arg组血清淀粉酶活性6h开始增高，24h达到高峰，6、12、24、48h血清淀粉酶活性与NS组比较差异有显著(P＜0.01)； (3)L-Arg组血清脂肪酶活性6h显著升高，12h达峰值，6h以后其活性与NS组比较差异均有统计意义(P＜0.01)； (4)L-Arg组胰腺湿／干比率6h升高，48h达峰值，6h以后其活性与NS组比较差异均有统计意义(P＜0.05)；
[Abstract]:Background: as a special type of acute pancreatitis, fulminant acute pancreattis (FAPP) is characterized by rapid onset of acute pancreatitis, severe onset, early multiple organ dysfunction and high mortality.Although the etiology and treatment of this kind of pancreatitis are often reported in clinic at present, there are few researches on FAP and the damage of extrapancreatic organs at home and abroad, and the understanding is extremely limited.Therefore, it is of great significance to establish FAP animal model, and then to systematically study the injury of FAP and extrapancreatic organs, and to reduce the death rate of clinical FAP.In this experiment, L-arginine L-arginine L-Arg was used as the inducer of AP to explore the establishment of rat FAP model by a new injection method.Methods one hundred and forty-four male Wistar rats were randomly divided into two groups: the experimental group (L-Arg group) and the control group (NS group (n = 72). The L-Arg group (n = 20) was injected intraperitoneally with arginine (n = 450 mg / 100 g).Once again, the NS group was injected with the same volume of normal saline in the same way.After the last injection, 30 rats in each group were taken to observe the mortality rate of rats in 0h-120h, 6 rats were killed at the corresponding time points of 0: 12, 24, 4872 and 120 hours, and the serum amylase, lipase, wet / dry ratio of pancreas, blood routine, liver function were observed.The pathological changes of pancreas, liver, kidney and lung were observed by HE staining at each time point above, and the pathological scores or ratings were given.Results the two groups were injected intraperitoneally and subcutaneously after 2 h interval.In the L-Arg group, 30 rats died for 6 hours and 0 rats died for 12 hours. One rat died at 24 hours. 7 rats died for 48 hours and 5 rats died for 72 hours. The total death rate was that 30 rats in 16/30=53.33%.NS group did not die within 120 hours.The activity of serum amylase in L-Arg group began to increase at 6h and reached the peak at 6121224h at 48h. There was a significant difference in serum amylase activity between group A and group NS (P < 0.01).The activity of serum lipase in the L-Arg group was significantly higher than that in the NS group at 6 h and reached the peak at 12 h and reached the peak level at 6 h. The difference was statistically significant compared with that in NS group (P < 0.01).The wet / dry ratio of pancreas in the L-Arg group was significantly higher than that in the NS group (P < 0.05).
【学位授予单位】：汕头大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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