人早期胚胎形态评分系统的建立和临床应用的研究

发布时间：2018-04-08 09:03

  本文选题：人早期胚胎　切入点：辅助生殖技术　出处：《第一军医大学》2005年硕士论文

【摘要】：目的:(1)探讨多个原核期形态学指标与Day3(取卵后第三天)胚胎着床之间的关系,验证并筛选能正确反映胚胎移植后种植潜能的原核期形态指标;(2)探讨多个Day3胚胎形态学指标和胚胎着床的关系,筛选能正确反映胚胎移植后种植潜能的Day3胚胎形态指标;(3)根据筛选的原核期和Day3胚胎形态观察指标,建立原核期胚胎评分、Day2(取卵后第二天)胚胎评分、Day3胚胎评分及Day3联合原核综合评分系统。 方法:前瞻性分析2004年10月～2005年1月,在南方医院生殖医学中心接受辅助生殖治疗的166位不孕患者。(1)166位不孕患者中,选取年龄均≤35岁,HCG日子宫内膜厚度均≥8mm,且Day3进行胚胎移植后100%植入的共30个移植周期患者(56个胚胎,植入组),同时随机选取满足上述条件的100%未植入32个移植周期患者(64个胚胎,未植入组)。根据原核期形态包括两原核是否相邻、大小、居于胞浆中的位置,核仁前体(Nucleolar precursor bodies,NPB)的排列方式、大小、数目,极体是否完整,胞浆晕的有无,胞质颜色及颗粒是否正常,胞质内空泡的有无进行数据统计,比较原核期形态与胚胎种植成功与否之间的相关性。(2)相同62个移植周期患者,根据Day3胚胎形态包括卵裂球数目、卵裂球大小差异、卵裂球核、卵裂球颜色及颗粒、碎片比例和碎片类型、胞质内空泡、透明带厚度及形状方面进行数据统计,比较Day3胚胎形态与胚胎种植成功与否之间的相关性。(3)根据上述实验结果,选取原核期两原核形态、
[Abstract]:Objective to study the relationship between the morphological indices of multiple prokaryotes and embryo implantation in Day3 (the third day after oviposition).To verify and screen the prokaryote morphological index which can correctly reflect the implantation potential after embryo transfer, and to explore the relationship between multiple Day3 embryo morphological indexes and embryo implantation.The morphological index of Day3 embryos, which can correctly reflect the implantation potential after embryo transfer, was screened according to the prokaryote stage and the morphological observation index of Day3 embryos.The embryo scoring system of day 2 (the second day after oviposition) and the embryo score of day 3 and Day3 combined with prokaryotic evaluation were established.Methods: from October 2004 to January 2005, 166 infertile patients received adjuvant reproductive treatment in the Center of Reproductive Medicine of Southern Hospital.A total of 30 patients (56 embryos) with 100% Day3 implantation after embryo transfer were selected. The endometrial thickness was 鈮，

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