EGF对乳腺干细胞体外增殖分化的影响及MSC体内移植的实验研究

发布时间：2018-04-09 22:06

本文选题：乳腺干细胞　切入点：MACS　出处：《吉林大学》2007年博士论文

【摘要】： 乳腺干细胞(mammary stem cells,MSCs)是一种存在于乳腺组织中的处于静止期的,未分化的细胞,并保持长期自我更新的能力,具有分化为乳腺组织特定形态特征和独特功能的各种类型细胞。乳腺干细胞的特点是不对称分裂,子代细胞保持干细胞特性或开始分化。干细胞还能对称分裂,两个子代细胞均为干细胞。这提示存在一种机制调节乳腺干细胞的自我更新能力与分化能力的平衡。乳腺干细胞可以分化为导管上皮细胞和肌上皮细胞。其细胞专一性的形态和各专一性的表现及功能特征都是细胞分化的结果。目前对于乳腺干细胞的研究需要找到能反应其细胞分化及代谢状况等精细变化特点的标志物,以及分离、纯化乳腺干细胞的技术手段和调控增殖与定向分化的各种体外培养体系等。本研究在第一部分实验中通过免疫磁珠分离技术分离出MUC-ESA+的成体乳腺干细胞,并在体外分离培养及传代,观察EGF对乳腺干细胞的增殖作用及乳腺干细胞表面标志物的变化。在第二部分实验中将其应用于裸鼠移植实验观察其在体内的增殖过程,从而为乳腺干细胞的进一步研究和临床应用打下基础。
[Abstract]:Mammary stem cells (MSCs) is a kind of cells in the mammary gland, which is in the stationary stage, undifferentiated, and maintains the ability of self-renewal for a long time. It has the ability to differentiate into the specific morphological features and unique functions of the mammary gland.Breast stem cells are characterized by asymmetric division, and progeny cells maintain stem cell identity or begin to differentiate.Stem cells also divide symmetrically, and both progeny cells are stem cells.This suggests that there is a mechanism to regulate the balance between self-renewal and differentiation of breast stem cells.Breast stem cells can differentiate into ductal epithelial cells and myoepithelial cells.The morphology of its cell specificity and the expression and functional characteristics of each specificity are the result of cell differentiation.At present, the study of mammary stem cells needs to find markers that can reflect the characteristics of cell differentiation and metabolism, as well as isolation.Methods of purifying mammary stem cells and in vitro culture systems for regulating proliferation and directional differentiation.In the first part of the study, the adult mammary stem cells of MUC-ESA were isolated by immunomagnetic bead separation, and cultured and subcultured in vitro to observe the proliferative effect of EGF on breast stem cells and the changes of surface markers of breast stem cells.In the second part of the experiment, it was applied to the nude mice transplantation experiment to observe its proliferation in vivo, so as to lay a foundation for the further study and clinical application of mammary stem cells.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2007
【分类号】：R329

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