广州管圆线虫cDNA文库的构建分析及广州管圆线虫病疗效考核抗原的筛选鉴定

发布时间：2018-04-10 02:24

本文选题：广州管圆线虫　切入点：cDNA文库　出处：《第一军医大学》2006年博士论文

【摘要】：广州管圆线虫病(Angiostrongyliasis)是由广州管圆线虫(Angiostrongylus cantonensis)感染而引起的。作为一种新发的食源性传染性疾病，广州管圆线虫病在全球的流行都呈现出上升的趋势，对人类的威胁也越来越大。广州管圆线虫主要侵犯中枢神经系统，，引起嗜中性粒细胞增多性脑膜炎或脑膜脑炎。感染该病后，绝大多数患者有头痛、肌肉痛、恶心、呕吐、感觉异常、触摸痛、发热、畏光、视力模糊、嗜睡、颈硬等症状。其中以头痛最突出，患者自诉“头痛欲裂”或忍受不了；全身疼痛、皮肤过敏的现象也比较严重；个别病人还会出现精神病样症状。我国是广州管圆线虫病的主要流行区之一，近几年的发病数逐渐增多，甚至出现了爆发流行。如何有效地防治广州管圆线虫病已成为亟待解决的重要问题。在广州管圆线虫病的防治工作中，诊断是关键。诊断明确，治疗及时，则病人的转归一般良好。但目前国内外都还缺乏敏感特异、能产业化生产、标准化的诊断试剂盒。针对目前这个难点问题，本论文进行了以下研究：一、广州管圆线虫幼虫cDNA文库的构建与幼虫、成虫基因表达模式分析鉴于广州管圆线虫在人体的感染和致病阶段主要为幼虫，本文构建了广州管圆线虫脑期幼虫的cDNA文库，并用EST(expressed sequence tag)方法对其基因表达谱和成虫的基因表达谱进行了初步分析，目的是对此新发传染病病原的分子生物学特征进行了解和为诊断性抗原的筛选提供库源。
[Abstract]:Angiostrongyliasis) was caused by Angiostrongylus cantonensis (Angiostrongylus cantonensis).As a new foodborne infectious disease, the prevalence of Angiostrongylus cantonensis in the world is on the rise, and the threat to human is increasing.Angiostrongylus cantonensis mainly invades the central nervous system, causing neutrophil meningitis or meningoencephalitis.After infection, most patients have headache, muscle pain, nausea, vomiting, abnormal sensation, touch pain, fever, photophobia, blurred vision, drowsiness, hard neck and other symptoms.Among them, the most prominent headache, the patient complained of "headache to crack" or can not endure; systemic pain, skin allergy phenomenon is more serious; individual patients will also have psychotic symptoms.China is one of the main endemic areas of Angiostrongylus cantonensis.How to effectively control Angiostrongylus cantonensis has become an important problem to be solved.Diagnosis is the key in the prevention and control of Angiostrongylus cantonensis.If the diagnosis is clear and the treatment is timely, the outcome of the patient is generally good.But at present, there is still a lack of sensitive and specific, industrial production, standardized diagnostic kit at home and abroad.Aiming at this difficult problem, this paper has carried out the following research: 1. Construction of cDNA library of Angiostrongylus cantonensis larvae and analysis of gene expression patterns of larvae and adultsIn view of the infection and pathogenicity of Angiostrongylus cantonensis is mainly larva, the cDNA library of Angiostrongylus cantonensis in brain stage was constructed, and the gene expression profile of Angiostrongylus cantonensis and the gene expression profile of adult nematode were preliminarily analyzed by EST(expressed sequence tagging method.The aim of this study was to understand the molecular biological characteristics of the pathogen of the new infectious disease and to provide a library source for the screening of diagnostic antigens.
【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R383

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