脊髓末端位置解剖及神经干细胞在终丝中分布研究

发布时间：2018-04-10 23:37

  本文选题：脊髓圆锥末端 + 顶臀长(CRL)　； 参考：《郑州大学》2005年硕士论文

【摘要】：目的 小儿脊髓栓系综合征(Tethered cord syndrome,TCS)是小儿外科常见疾病。目前诊断仍沿用欧洲人的诊断标准,即脊髓圆锥末端位于L_3以下并出现相应的临床症状。治疗主要是手术松解,但仍有不少患儿疗效并不满意。近年对亚洲人脊髓圆锥末端位置研究,结果发现亚洲人脊髓圆锥末端位置与欧洲人之间存在争议。神经干细胞研究进展给该病带来希望。本实验以探索国人胎儿新生儿脊髓圆锥末端位置,以便对胎儿新生儿期脊髓栓系或脊髓栓系综合征早期作出诊断及指导腰部脑脊液穿刺、麻醉等操作;研究儿童终丝中神经干细胞(Neural stem cells,NSCs)的分布,为脊髓栓系综合征及其它中枢神经系统疾病的治疗提供新的材料。 材料和方法 1.对124例胎儿和死亡足月新生儿进行解剖,游离出第十二肋及各椎体横突作为标记,测量项臀长(crown—rump length,CRL),按Vettivel分组方法共分Ⅷ组。脊髓圆锥末端确定为:最后一对神经根连接处水平。圆锥末端位于横突基部上缘时表示在椎体上缘、位于横突基部下缘时表示在椎体中点、圆锥末端位于两横突之间表示在椎体下缘、上下缘之间对应的为椎间盘。所得结果进行统计学处理。 2.取TCS患者内终丝近远端40例,用免疫组织化学的方法进行染色,观察神经上皮干细胞蛋白(Nestin)表达。 结果
[Abstract]:PurposeTethered cord syndrome is a common disease in pediatric surgery.At present, the European diagnostic criteria are still used, that is, the end of the spinal cones is located below L\ +\ +\ {\}\ {\}\ ^\\\The main treatment is surgical release, but there are still many children with unsatisfactory results.In recent years, we have studied the terminal position of the spinal cones in Asian people and found that there is a dispute between the terminal position of the spinal cones in Asians and the Europeans.The progress of neural stem cell research brings hope to the disease.The purpose of this study was to explore the terminal position of spinal cord cones in Chinese fetus, so as to diagnose the tethered cord or tethered cord syndrome early and to guide the operation of lumbar cerebrospinal fluid puncture and anaesthesia.To study the distribution of neural stem cells in terminal filaments of children, and to provide new materials for the treatment of tethered cord syndrome and other central nervous system diseases.Materials and methods1.124 fetuses and dead full-term newborns were dissected. The twelfth rib and the transverse process of vertebrae were separated as markers to measure the length of nail-buttock crown-rump length of CRLL. According to the method of Vettivel grouping, they were divided into 鈪，

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