恒河猴颈段脊髓半横断损伤模型的建立

发布时间：2018-04-12 22:06

本文选题：脊髓损伤 + 动物模型　；参考：《中国人民解放军军医进修学院》2006年硕士论文

【摘要】：脊髓损伤(spinal cord injury,SCI)是临床常见创伤,其致残率与致死率高,一直是基础和临床医学的研究热点。近年来,为探讨脊髓损伤修复的机理进行了大量的实验研究取得许多有价值的实验结果,同时,动物模型研究也取得了进一步的完善,多种类型的脊髓损伤模型广泛应用于基础研究中,但在不同种类动物实验中评价脊髓损伤存在很大差异。建立脊髓半横断模型,为研究脊髓损伤后神经修复及运动功能改善的研究提供良好的实验模型,才有可能深入探讨脊髓损伤修复及再生机理,使其基础研究得到新的突破,为临床治疗提供有价值的实验数据。作者用灵长类恒河猴作为实验动物,在一定程度上成功模拟了人类脊髓损伤后的病理改变和临床表现,建立标准可重复的模型。实验方法:全麻的恒河猴颈部后正中切口显露C3-5椎体平面脊髓,于C4用虹膜刀切开脊髓左半侧0.5cm,通过体感诱发电位(somatosensory evoked potential SEP)监测确定波幅降低50%,术后24h、7d重新测量SEP;观察肢体运动情况,进行运动评分、用数码相机录像分析肢体活动构成。结果表明:术后24h、7d作为观察时间窗,肢体运动评分由术前的6分降到1分。SEP显示前肢正中神经术前潜伏期为15.54±2.69 ms,术后24h为22.36±4.19ms、7d为23.14±3.89ms,延长明显(P0.01)。术前波幅为13.04±4.95μv,术后24h为5.28±3.02μV、7d为5.32±2.75μV,降低明显(P0.01)。后肢胫后神经术前潜伏期22.84±3.59 ms;术后24h为33.02
[Abstract]:Spinal cord injury (sci) is a common clinical trauma with high disability rate and fatality rate. It has always been a hot topic in basic and clinical medicine.In recent years, in order to explore the mechanism of spinal cord injury repair, a large number of experimental studies have been carried out to obtain many valuable experimental results, at the same time, the animal model research has been further improved.Many types of spinal cord injury models are widely used in basic research, but there are great differences in the evaluation of spinal cord injury in different kinds of animal experiments.To provide valuable experimental data for clinical treatment.Using primate rhesus monkey as experimental animal, the pathological changes and clinical manifestations of human spinal cord injury were successfully simulated to a certain extent, and a standard repeatable model was established.Methods: the spinal cord of C3-5 vertebra was exposed through the median incision of the neck of rhesus monkey under general anesthesia.The left half of spinal cord was cut with iris knife at 0.5 cm. Somatosensory evoked potential (SEP) was monitored to determine the decrease of amplitude by 50%, and then to re-measure SEP 24 hours after operation, to observe the movement of limbs, to score the motion, and to analyze the composition of limb motion by digital camera video recording.The amplitude of the wave was 13.04 卤4.95 渭 v before operation and 5.28 卤3.02 渭 V at 24 hours after operation. The amplitude was 5.32 卤2.75 渭 V on the 7th day after operation.The latency of posterior tibial nerve in hind limb was 22.84 卤3.59 Ms before operation and 33.02 in 24 hours after operation.
【学位授予单位】：中国人民解放军军医进修学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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