模拟自然条件下湖沼型和山区型钉螺存活率及其血吸虫易感性研究

发布时间：2018-04-14 20:03

  本文选题：血吸虫 + 钉螺　； 参考：《南昌大学学报(医学版)》2014年12期

【摘要】：目的对比江西星子地域株湖沼型肋壳钉螺、江西玉山地域株山区型肋壳钉螺和四川彭山地域株山区型光壳钉螺在模拟江西省星子县野外自然条件下放养4个月的存活率及其对同种血吸虫的易感性差异。方法野外采集江西省星子地域株湖沼型肋壳钉螺、玉山地域株山区型肋壳钉螺和四川省彭山地域株山区型光壳钉螺,采用逸蚴法筛选出阴、阳性钉螺,用星子地域株阳性钉螺感染家兔,45 d后取家兔肝脏并获得毛蚴,在26~30℃室温和日光灯的照射下,以毛蚴数量与钉螺数量20∶1进行人工感染。感染后,将钉螺置于江西省星子县血吸虫病防治站进行室外模拟现场饲养,4个月后回收钉螺,对比存活率,并采用逸蚴法对比其感染率。结果江西玉山山区型、四川彭山山区型钉螺均能在江西省星子县模拟自然条件下存活,但其存活率较江西星子湖沼型钉螺显著降低(P0.001);江西星子湖沼型钉螺和江西玉山山区型钉螺易感染江西星子地区血吸虫毛蚴,且前者的感染率明显高于后者(P0.001),四川彭山山区型钉螺不易感染该血吸虫毛蚴。结论山区型钉螺可以在湖沼地区存活,其存活率与湖沼型钉螺不同;湖沼型钉螺和山区型钉螺对同种血吸虫的易感性不同。
[Abstract]:Objective to compare hupperus hupensis with lake-type ribbed shell in Xingzi region of Jiangxi Province.The survival rate and susceptibility to Schistosoma japonicum of hind snails of mountain type in Yushan region of Jiangxi Province and that of light shell snail species in mountain area of Pengshan region in Sichuan province were studied under simulated natural conditions in Xingzi county Jiangxi Province for 4 months.Methods Oncomelania hupensis was collected from Xingzi region, Jiangxi Province, Yushan region, Yushan region and Pengshan region, Sichuan Province, respectively. The negative and positive snails were screened by metacercosis method.After 45 days of positive snail infection, the liver of the rabbit was taken and the cercariae were obtained. Under the irradiation of 2630 鈩，

本文编号：1750809